Non-invasive method to assess genotoxicity of nocodazole interfering with spindle formation in mammalian oocytes.

Shen Y; Betzendahl I; Sun F; Tinneberg HR; Eichenlaub Ritter U

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Non-invasive method to assess genotoxicity of nocodazole interfering with spindle formation in mammalian oocytes.

机译：评估诺考达唑干扰哺乳动物卵母细胞纺锤体形成的遗传毒性的非侵入性方法。

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Trisomies due to nondisjunction in oogenesis are still a major cause of genetic diseases in humans. In this study, we analysed spindle morphology of in vitro matured nocodazole-exposed mouse oocytes by novel non-invasive Polscope-microscopy, and compared images to those obtained by anti-tubulin immunofluorescence of fixed oocytes. Polscope revealed a reduction in the numbers of oocytes expressing a birefringent spindle, and alterations in spindle morphology at concentrations of nocodazole below those inducing detectable aberrations in immunofluorescence. Hyperploidy increased significantly at a concentration of 40nM nocodazole in mouse metaphase II oocytes, similar to thresholds inducing nondisjunction in cultured human lymphocytes. In conclusion, Polscope represents a novel highly sensitive, non-invasive method to identify chemicals inducing severe spindle aberrations that predispose mammalian oocytes to nondisjunction. Polscope may provide information on the functionality of the spindle in experimental studies but is also compatible with clinical trials in human assisted reproduction due to its non-invasive nature.

机译：由于卵子发生不分离而引起的三体性仍然是人类遗传疾病的主要原因。在这项研究中，我们通过新型非侵入性Polscope显微镜分析了体外成熟的暴露于诺考达唑的小鼠卵母细胞的纺锤形，并将图像与通过固定卵母细胞的抗微管蛋白免疫荧光法获得的图像进行了比较。 Polscope揭示了表达双折射纺锤体的卵母细胞数量减少，并且在诺考达唑浓度低于诱导免疫荧光中可检测到畸变的浓度时纺锤体形态发生了变化。在小鼠中期II卵母细胞中，在40nM诺考达唑浓度下，超倍性显着增加，类似于在培养的人类淋巴细胞中诱导不分离的阈值。总之，Polscope代表了一种新型的高度灵敏，非侵入性的方法，可以识别导致严重卵轴畸变的化学物质，这些畸变使哺乳动物卵母细胞易于分离。 Polscope可在实验研究中提供有关纺锤体功能的信息，但由于其非侵入性，也可与人工辅助生殖的临床试验兼容。

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《Reproductive toxicology》 |2005年第4期|共13页
作者
Shen Y; Betzendahl I; Sun F; Tinneberg HR; Eichenlaub Ritter U;
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正文语种 eng
中图分类药学;
关键词
Nondisjunction; Genetic; Mammals; Science of Morphology; novel; genotoxicity; 不分离; 遗传; 哺乳动物;

机译：Nondisjunction;Genetic;Mammals;Science of Morphology;novel;genotoxicity;不分离;遗传;哺乳动物;

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1. Non-invasive method to assess genotoxicity of nocodazole interfering with spindle formation in mammalian oocytes. [J] . Shen Y, Betzendahl I, Sun F, Reproductive toxicology . 2005,第4期

机译：评估诺考达唑干扰哺乳动物卵母细胞纺锤体形成的遗传毒性的非侵入性方法。
2. NON-INVASIVE METHODS TO ASSESS CO-OCCURRENCE OF MAMMALIAN CARNIVORES [J] . Reed Sarah E. . The Southwestern Naturalist . 2011,第2期

机译：评估哺乳动物食肉同生的非侵入性方法
3. NEDD1 is crucial for meiotic spindle stability and accurate chromosome segregation in mammalian oocytes. [J] . Ma W, Baumann C, Viveiros MM Developmental biology . 2010,第2期

机译：NEDD1对于哺乳动物卵母细胞的减数分裂纺锤体稳定性和准确的染色体分离至关重要。
4. Non-Invasive Methods for Measurement of Carotenoids in Mammalian Skin [C] . Maxim E. Darvin, Juergen Lademann Asia Communications and Photonics Conference . 2014

机译：非侵入性方法测量哺乳动物皮肤中的类胡萝卜素
5. Epithelial Geometry Regulates Spindle Orientation and Progenitor Fate During Formation of the Mammalian Epidermis [D] . Box, Kimberly Simone. 2019

机译：上皮几何形状在形成哺乳动物表皮时调节主轴取向和祖先命运
6. Ablation of PRC1 by Small Interfering RNA Demonstrates that Cytokinetic Abscission Requires a Central Spindle Bundle in Mammalian Cells whereas Completion of Furrowing Does Not [O] . Cristiana Mollinari, Jean-Philippe Kleman, Yasmina Saoudi, 2005

机译：小干扰RNA对PRC1的消融表明细胞动力学的脱落需要哺乳动物细胞中的中心纺锤束而犁沟的完成不需要
7. Supplemental Information 7: The effect of inactive CORM-2 (iCORM-2) and inactive CORM-A1 (iCORM-A1) on the meiotic maturation of porcine oocytes. The data were used to assess the effect of inactive CORM-2 and inactive CORM-A1 on the meiotic maturation of porcine oocytes. [O] . -1

机译：补充信息7：非活性CORM-2（ICOM-2）和非活性CORM-A1（ICOM-A1）对猪卵母细胞的减数分裂成熟的影响。该数据用于评估非活性CINM-2和非活性COMM-A1对猪卵母细胞的减数分裂成熟的影响。
8. Strategies for the Use of Computational SAR Methods in Assessing Genotoxicity [R] . Richard, A. M., Rabinowitz, J. R., Waters, M. D. 1989

机译：利用计算saR方法评估遗传毒性的策略

Non-invasive method to assess genotoxicity of nocodazole interfering with spindle formation in mammalian oocytes.

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