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首页> 外文期刊>Reproductive toxicology >Bisphenol A exposure leads to specific microRNA alterations in placental cells.
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Bisphenol A exposure leads to specific microRNA alterations in placental cells.

机译：双酚A暴露导致胎盘细胞中特定的microRNA改变。

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摘要

Exposure to bisphenol A (BPA) has been observed to alter developmental pathways and cell processes, at least in part, through epigenetic mechanisms. This study sought to investigate the effect of BPA on microRNAs (miRNAs) in human placental cells. miRNA microarray was performed following BPA treatment in three immortalized cytotrophoblast cell lines and the results validated using quantitative real-time PCR. For functional analysis, overexpression constructs were stably transfected into cells that were then assayed for changes in proliferation and response to toxicants. Microarray analysis revealed several miRNAs to be significantly altered in response to BPA treatment in two cell lines. Real-time PCR results confirmed that miR-146a was particularly strongly induced and its overexpression in cells led to slower proliferation as well as higher sensitivity to the DNA damaging agent, bleomycin. Overall, these results suggest that BPA can alter miRNA expression in placental cells, a potentially novel mode of BPA toxicity.
机译：已观察到双酚A（BPA）的暴露至少部分通过表观遗传机制改变了发育途径和细胞过程。这项研究试图调查BPA对人胎盘细胞中microRNA（miRNA）的影响。 BPA处理后，在三种永生化细胞滋养层细胞系中进行了miRNA微阵列分析，并使用定量实时PCR验证了结果。为了进行功能分析，将过表达构建体稳定转染到细胞中，然后分析其增殖和对毒物的反应变化。微阵列分析显示，在两种细胞系中，响应BPA处理，一些miRNA显着改变。实时PCR结果证实，miR-146a被特别强烈地诱导，其在细胞中的过表达导致增殖减慢以及对DNA破坏剂博来霉素的敏感性更高。总体而言，这些结果表明，BPA可以改变胎盘细胞中的miRNA表达，这是BPA毒性的一种潜在的新模式。

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