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首页> 外文期刊>Cellular Signalling >Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: Identification of BRI3 and HSF2 as novel targets
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Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: Identification of BRI3 and HSF2 as novel targets

机译：使用SAGE，全基因组微阵列和启动子分析对Wnt / B-catenin / TCF4途径进行分析：鉴定BRI3和HSF2作为新靶标

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The Wnt signaling pathway is involved in many differentiation events during embryonic development and can lead to tumor formation after aberrant activation of its components. β-catenin, a cytoplasmic component, plays a major role in the transduction of canonical Wnt signaling. The aim of this study was to identify novel genes that are regulated by active β-catenin/TCF signaling in hepatocellular carcinoma-derived Huh7 cells with high (transfected) and low β-catenin/TCF activities. High TCF activity Huh7 cells led to earlier and larger tumor formation when xenografted into nude mice. SAGE (Serial Analysis of Gene Expression), genome-wide microarray and in silico promoter analysis were performed in parallel, to compare gene expression between low and high β-catenin/TCF activity clones, and also those that had been rescued from the xenograft tumors. SAGE and genome-wide microarray data were compared and contrasted. BRI3 and HSF2 were identified as novel targets of Wnt/β-catenin signaling after combined analysis and confirming experiments including qRT-PCR, ChIP, luciferase assay and lithium treatment.
机译：Wnt信号通路参与胚胎发育过程中的许多分化事件，并可能在其组分异常激活后导致肿瘤形成。 β-catenin是一种细胞质成分，在经典Wnt信号转导中起主要作用。这项研究的目的是在肝细胞癌衍生的具有高（转染）和低β-catenin/ TCF活性的Huh7细胞中鉴定受活性β-catenin/ TCF信号调节的新基因。当异种移植到裸鼠体内时，高TCF活性的Huh7细胞导致更早和更大的肿瘤形成。并行进行了SAGE（基因表达的序列分析），全基因组微阵列和计算机启动子分析，以比较低和高β-catenin/ TCF活性克隆以及从异种移植肿瘤中拯救出来的克隆之间的基因表达。 。 SAGE和全基因组微阵列数据进行了比较和对比。经过组合分析和包括qRT-PCR，ChIP，荧光素酶测定和锂处理在内的实验确认后，BRI3和HSF2被确定为Wnt /β-catenin信号传导的新靶标。

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