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首页> 外文期刊>Reproductive toxicology >Orthotopic transplantation of neonatal GFP rat ovary as experimental model to study ovarian development and toxicology.
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Orthotopic transplantation of neonatal GFP rat ovary as experimental model to study ovarian development and toxicology.

机译：原位移植新生GFP大鼠卵巢作为实验模型，研究卵巢发育和毒理学。

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摘要

The rat is one of the most commonly used experimental animal species in biomedical research. The availability of new research tools in rats could therefore provide considerable advances in the areas where this mammal is extensively used. We report the development of a new green fluorescent protein (GFP) rat strain suitable for organ transplantation and the birth of GFP rats following orthotopic transplantation of neonatal ovaries from this newly developed GFP rat strain to a wild-type Fischer 344 (F344) strain. A new GFP rat strain was developed by backcrossing eGFP Sprague-Dawley (SD-Tg(CAG-EGFP)Cz-004Osb) to wild-type F344 for eight generations. Whole ovaries from postnatal day (PND) 8 or PND 21 GFP rats were transplanted orthotopically to bilaterally ovariectomized wild-type adult females (n=6). All recipients were mated, and three of the five resulting litters contained GFP pups. In the PND 8 group, all recipients cycled regularly and the ovarian morphology appeared normal when collected at 9 months post-transplantation. In the PND 21 group, 60% of the recipients displayed regular estrous cycles at 9 months post-transplantation, but showed reduced ovarian size. This new strain and neonatal orthotopic transplantation could be useful for many biomedical fields including transplantation, development, and reproductive toxicology.
机译：大鼠是生物医学研究中最常用的实验动物之一。因此，在大鼠中可获得新的研究工具可能会在广泛使用该哺乳动物的领域中提供可观的进步。我们报告了一种适合器官移植的新绿色荧光蛋白（GFP）大鼠菌株的开发，以及从这种新开发的GFP大鼠菌株原位移植新生卵巢到野生型Fischer 344（F344）菌株后，原位移植了新生大鼠的GFP大鼠。通过将eGFP Sprague-Dawley（SD-Tg（CAG-EGFP）Cz-004Osb）回交至野生型F344八代来开发新的GFP大鼠品系。将来自产后一天（PND）8或PND 21 GFP大鼠的整个卵巢原位移植到双侧卵巢切除的野生型成年雌性动物中（n = 6）。所有接受者均已交配，并在五个产仔中的三个含有GFP幼仔。在PND 8组中，所有受体均定期循环，并且在移植后9个月收集时卵巢形态似乎正常。在PND 21组中，有60％的接受者在移植后9个月表现出规律的发情周期，但卵巢尺寸减小。这种新菌株和新生儿原位移植可用于许多生物医学领域，包括移植，发育和生殖毒理学。

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