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首页> 外文期刊>Russian Journal of Plant Physiology >Low-temperature and substrate induction of the gene for Delta 9 fatty acid desaturase in the thermophilic cyanobacterium Synechococcus vulcanus
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Low-temperature and substrate induction of the gene for Delta 9 fatty acid desaturase in the thermophilic cyanobacterium Synechococcus vulcanus

机译：嗜热蓝藻Syechococcus vulcanus中Delta 9脂肪酸去饱和酶基因的低温和底物诱导

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摘要

A decrease in ambient temperature induced the desC gene encoding the only acyl-lipid desaturase of thermophilic cyanobacterium Synechococcus vulcanus. The addition of the substrate for this enzyme, stearic acid (18:0), to the culture medium of cyanobacterium grown at optimum temperature also induced the desC gene. At 55 and 45degreesC, gene transcription started at the position -59 upstream of the translation initiation codon. After addition of 18:0, two specific pools of transcripts were detected. Some transcripts corresponded in size to transcripts synthesized at 55 and 45degreesC, and other transcripts were longer. The determination of sites of the transcript synthesis initiation showed that 18:0 additionally induced transcription from positions -163 and -169. These additional initiation sites were positioned within the coding sequence of the hemN gene preceding the desC gene. Thus, at low-temperature and substrate induction, the expression of the desC gene was evidently controlled by different promoter sequences.
机译：环境温度的降低诱导了desC基因的编码，该基因编码嗜热性蓝藻硫化膜球菌的唯一酰基-脂质去饱和酶。将这种酶的底物硬脂酸（18：0）添加到在最佳温度下生长的蓝细菌的培养基中，也诱导了desC基因。在55和45°C，基因转录在翻译起始密码子上游-59位置开始。添加18：0后，检测到两个特定的转录本库。一些成绩单的大小对应于在55和45℃合成的成绩单，而其他成绩单则更长。转录物合成起始位点的确定表明18：0另外诱导了从位置-163和-169的转录。这些另外的起始位点位于desC基因之前的hemN基因的编码序列内。因此，在低温和底物诱导下，desC基因的表达显然受到不同启动子序列的控制。

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