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首页> 外文期刊>Russian Journal of Plant Physiology >Statistical parameters reflecting morphogenetic capacity of soft spring wheat calluses
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Statistical parameters reflecting morphogenetic capacity of soft spring wheat calluses

机译：反映软春小麦老茧形态形成能力的统计参数

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摘要

Callus cultures of soft spring wheat were subcultured without separation into explants to follow the line "one excised embryo-one callus". This approach revealed the following statistical correlations. Within every cultivar of Triticum aestivum L. and within a row of cultivars arranged in ascending order according to the frequency of embryogenic callus formation, positive correlations (at P = 95) were found between the proliferative activity of callus cells and the frequency of embryogenic callus formation. A reliable intraspecies correlation (significant at P = 95) between multiple regenerations of plants from calluses and the tillering trait (bushiness) of donor plants was also found. We assessed the importance of various statistical parameters of callus cultures for preliminary estimation of morphogenesis efficiency at early stages of culturing. Frequencies of callusogenesis and the growth curves for randomly selected calluses turned out to be noninformative characteristics, unless the morphogenetic activity of calluses was taken into account. The following statistical parameters were found to correlate with the morphogenetic capacity of wheat calluses: gradually increasing coefficients of variation in fresh weight of primary calluses, a larger callus size, and higher fresh weight gain in potentially morphogenetic calluses.
机译：将软春小麦的愈伤组织培养物传代培养，不分离成外植体，遵循“一个切除的胚胎-一个愈伤组织”的路线。这种方法揭示了以下统计相关性。在每个普通小麦的品种中，以及根据胚发生愈伤组织形成的频率按升序排列的一排品种中，在愈伤组织细胞的增殖活性与胚发生愈伤组织的频率之间发现正相关（P = 95）。编队。还发现了愈伤组织的多次再生与供体植物的分er性状（灌木度）之间存在可靠的种内相关性（在P = 95时显着）。我们评估了愈伤组织培养物的各种统计参数对于培养初期阶段形态发生效率的初步估计的重要性。除非考虑到愈伤组织的形态发生活性，否则随机选择的愈伤组织的愈伤组织发生频率和生长曲线被证明是非信息性特征。发现以下统计参数与小麦愈伤组织的形态发生能力相关：逐渐增加的初级愈伤组织鲜重变异系数，较大的愈伤组织大小和潜在的形态发生愈伤组织中较高的鲜重增重。

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