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首页> 外文期刊>Russian Journal of Plant Physiology >Ethephon- and jasmonate-elicited pathogenesis-related ribonucleases in cultured ginseng cells
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Ethephon- and jasmonate-elicited pathogenesis-related ribonucleases in cultured ginseng cells

机译:栽培人参细胞中乙烯利和茉莉酸引起的发病相关核糖核酸酶

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摘要

Cultured ginseng cells (Panax ginseng C.A. Mey strain R-1) produce two proteins exhibiting RNase activity (Pg1 and Pg2), which, on the basis of their amino acid sequences, have been earlier referred to intracellular pathogenesis-related proteins. An immunoenzyme technique for estimation of these proteins was developed. A close correlation was found between the content of these proteins and the RNase activity of the cultured cells. Ethephon and jasmonic acid activated the RNase activity, ethephon being more efficient. Salicylic acid did not activate Pg1 and Pg2; high concentrations of salicylic acid suppressed the RNase activity of the culture. The protein kinase inhibitor, H-7, reduced the content and activity of RNases both in the presence and absence of ethephon. The results obtained permit a suggestion that ethylene and jasmonic acid signaling pathways, which include protein phosphorylation, are involved in the induction of PR-10 proteins.
机译:培养的人参细胞(Panax ginseng C.A. Mey菌株R-1)产生两种具有RNase活性的蛋白(Pg1和Pg2),根据其氨基酸序列,这些蛋白先前被称为胞内发病相关蛋白。开发了用于估计这些蛋白质的免疫酶技术。发现这些蛋白质的含量与培养细胞的RNase活性密切相关。乙烯利和茉莉酸激活了RNase活性,乙烯利效率更高。水杨酸不能激活Pg1和Pg2;高浓度的水杨酸抑制了培养液的RNase活性。在存在和不存在乙烯利的情况下,蛋白激酶抑制剂H-7均可降低RNase的含量和活性。获得的结果表明,乙烯和茉莉酸信号传导途径(包括蛋白质磷酸化)参与了PR-10蛋白的诱导。

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