Immunoenzyme analysis of cytokinins as an assay for cytokinin oxidase activity

Veselov SY; Simonyan MV

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Immunoenzyme analysis of cytokinins as an assay for cytokinin oxidase activity

机译：细胞分裂素的免疫酶分析，作为细胞分裂素氧化酶活性的测定

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A new highly sensitive method of measuring cytokinin oxidase activity was worked out on the basis of an immunoenzyme test-system for isopentenyladenosine (IPA) assay. The enzyme activity is determined by immunoenzyme assay as the difference between the IPA quantities in the reaction mixture before and after incubation. The specificity of the assay was verified by using the well-known enzyme activators and inhibitors and by monitoring the formation of other cytokinins.

机译：在异戊烯基腺苷（IPA）检测的免疫酶测试系统的基础上，制定了一种新的高度敏感的测量细胞分裂素氧化酶活性的方法。通过免疫酶测定法将酶活性确定为温育前后反应混合物中IPA量之间的差异。通过使用众所周知的酶激活剂和抑制剂并监测其他细胞分裂素的形成，验证了测定的特异性。

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来源
《Russian Journal of Plant Physiology》 |2004年第2期|共5页
作者
Veselov SY; Simonyan MV;
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正文语种 eng
中图分类植物学;
关键词
Zea mays; Cytokinin oxidase; Immunoenzyme analysis; Zea-mays; Purification; Maize; Gene; Dna;

机译：玉米;细胞分裂素氧化酶;免疫酶分析;玉米-玉米;纯化;玉米;基因;脱氧核糖核酸;

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1. Immunoenzyme analysis of cytokinins as an assay for cytokinin oxidase activity [J] . Veselov SY, Simonyan MV Russian Journal of Plant Physiology . 2004,第2期

机译：细胞分裂素的免疫酶分析，作为细胞分裂素氧化酶活性的测定
2. Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity, Distribution, and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins [J] . von Schwartzenberg Klaus, Ncclez Marta FerncLndez, Blaschke Hanna, Plant physiology . 2007,第3期

机译：苔藓植物Physcomitrella patens中的细胞分裂素：活性，分布和细胞分裂素氧化酶/脱氢酶的过表达分析揭示了细胞外细胞分裂素的作用
3. Cytokinins in the bryophyte Physcomitrella patens: Analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins [J] . von Schwartzenberg K, Nunez MF, Blaschke H, Plant physiology . 2007,第3期

机译：苔藓植物Physcomitrella patens中的细胞分裂素：活性，分布和细胞分裂素氧化酶/脱氢酶过表达的分析揭示了细胞外细胞分裂素的作用
4. Antiproliferattve and growth-stimulating activities of synthetic cytokinin analogs [C] . F. FRANEK, M. STRNAD, L. HAVLICEK, Annual Meeting of the Japanese Association for Animal Cell Technology . 2002

机译：抗脂素和生长刺激的合成细胞蛋白类似物的活性
5. Ribonuclease and cytokinin modulating activities of pathogenesis-related (PR) 10 proteins in plant responses to abiotic stresses. [D] . Srivastava, Sanjeeva. 2006

机译：植物对非生物胁迫的应答中，病原相关（PR）10蛋白的核糖核酸酶和细胞分裂素调节活性。
6. Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity Distribution and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins [O] . Klaus von Schwartzenberg, Marta Fernández Núñez, Hanna Blaschke, 2007

机译：苔藓植物Physcomitrella patens中的细胞分裂素：活性分布和细胞分裂素氧化酶/脱氢酶的过表达分析揭示了细胞外细胞分裂素的作用
7. Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity, Distribution, and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins1[W] [O] . von Schwartzenberg, Klaus, Núñez, Marta Fernández, Blaschke, Hanna, 2007

机译：苔藓植物Physcomitrella patens中的细胞分裂素：活性，分布和细胞分裂素氧化酶/脱氢酶过表达的分析揭示了细胞外细胞分裂素的作用[W]

Immunoenzyme analysis of cytokinins as an assay for cytokinin oxidase activity

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