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首页> 外文期刊>Russian Journal of Plant Physiology >Characteristics of photosystem I complexes in the end membranes of pea granal thylakoids
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Characteristics of photosystem I complexes in the end membranes of pea granal thylakoids

机译：豌豆类囊体端膜中光系统I配合物的特征

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摘要

Two fractions of the light fragments enriched in the photosystem I (PSI) complexes were obtained from pea (Pisum sativum L.) thylakoids by digitonin treatment and subsequent differential centrifugation. The ratio of chlorophyll a to chlorophyll b, chlorophyll/P700 spectra of low-temperature fluorescence, and excitation spectra of long-wave fluorescence were measured. These characteristics were shown to be different due to variation in the size and composition of the light-harvesting antenna of PSI complexes present in the particles obtained. The larger antenna size of one of the fractions was related to the incorporation of the pool of light-harvesting complex 11 (LHCII). A comparison with the data available allowed us to identify these particles as fragments of intergranal thylakoids and end membranes of granal thylakoids. The suggestion that an increase in the PSI light-harvesting antenna in intergranal thylakoids is related to the attachment of phosphorylated LHCII is discussed.
机译：富集了光系统I（PSI）复合物的轻片段的两部分是通过豆黄素处理和随后的离心分离从豌豆类豌豆类囊体获得的。测定叶绿素a与叶绿素b的比例，低温荧光的叶绿素/ P700光谱和长波荧光的激发光谱。由于存在于获得的颗粒中的PSI复合物的光收集天线的尺寸和组成的变化，这些特性显示出不同。其中一部分的较大天线尺寸与光收集复合物11（LHCII）库的合并有关。与可用数据的比较使我们能够将这些颗粒鉴定为粒间类囊体和粒状类囊体终膜的碎片。讨论了粒间类囊体中PSI捕光触角的增加与磷酸化LHCII的附着有关的建议。

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