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首页> 外文期刊>Russian Journal of Plant Physiology >The effect of seed cryopreservation on the development of protocorms by the hybrid orchid Bratonia
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The effect of seed cryopreservation on the development of protocorms by the hybrid orchid Bratonia

机译：种子低温保存对杂交兰花Bratonia发育原球茎的影响

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The aim of this work was to study the influence of storage in liquid nitrogen on the viability of seeds of the hybrid orchid Bratonia and further development of its protocorms in vitro. Seeds were frozen in ampoules by direct immersion in liquid nitrogen and stored in the cryobank for a month. The germination rates of cryopreserved and control (nonfrozen) seeds did not differ and remained as high as 100%. The protocorms derived were cultured on the agar-solidified Murashige and Skoog nutrient medium (MS), half-strength MS and Knop media and also in Morel liquid medium. During the first 45 days of culturing, protocorms derived from cryopreserved seeds grew faster than control protocorms on the MS and half-strength MS media but, at longer culturing (496 days), the size of control protocorms was significantly larger. After 639 days of culturing, there was no difference in the amount of perished, budding, and newly formed protocorms obtained from cryopreserved and control seeds, except half-strength MS medium where the number of budding protocorms in the case of cryopreserved seeds was a little greater than in the control treatment. After seed cryopreservation, the frequency of budding and newly formed protocorms was greater on the agarized MS and in liquid Morel media. Cryopreservation had little effect on the subsequent growth of protocorms in vitro. The preferable nutrient media for culturing the protocorms have been suggested.
机译：这项工作的目的是研究液氮中的储存对杂交兰花Bratonia种子的生存能力及其体外原球茎的进一步发展的影响。通过直接浸入液氮中将种子冷冻在安瓿中，并在冷冻库中保存一个月。低温保存和对照（非冷冻）种子的发芽率没有差异，仍高达100％。衍生的原球茎在琼脂固化的Murashige和Skoog营养培养基（MS），半强度MS和Knop培养基以及羊肚菌液体培养基中培养。在培养的前45天中，在MS和半强度MS培养基上，低温保存的种子衍生的原球茎比对照原球茎生长快，但在更长的培养时间（496天）中，对照原球茎的大小明显更大。培养639天后，从低温保存和对照种子获得的枯萎，出芽和新形成的原球茎的数量没有差异，除了半强度MS培养基外，对于低温保存的种子，出芽的原球茎的数量很少大于对照治疗。种子冷冻保存后，琼脂化的MS和液体羊肚菌培养基中出芽和新形成的原球茎的发生频率更高。冷冻保存对原球茎在体外的后续生长几乎没有影响。已经提出了用于培养原球茎的优选的营养培养基。

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