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首页> 外文期刊>Cereal Chemistry >Fungal Susceptibility at Four Temperature-Moisture Combinations and Carbon Dioxide Kit Color Reader Evaluation
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Fungal Susceptibility at Four Temperature-Moisture Combinations and Carbon Dioxide Kit Color Reader Evaluation

机译：四种温湿组合下的真菌敏感性和二氧化碳试剂盒颜色读取器评估

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摘要

The susceptibility of shelled corn to invasion by storage fungi was assessed using a carbon dioxide (CO2) test kit. Shelled corn samples were rewetted to either 16 or 21% wb moisture content (MC) and incubated <= 80 hr at either 24 or 30 degrees C in sealed glass jars. CO2 concentration in the jars was quantified using the color number (0-5) of an indicator gel, which was determined either by visual comparison to a color card (VR) or using a Digital Color Reader (DCR). Plots of DCR color numbers versus time were smoother than those for VR color numbers. The DCR was better able to distinguish differences among samples, especially when color numbers were <2.0. The highest coefficients of determination (r(2)) for linear regressions of color number versus ergosterol difference were 0.75 for the DCR readings and 0.73 for the VR readings. Rankings of fungal susceptibility were similar at all four temperature-MC combinations and many r(2) values for linear regressions comparing readings at other conditions with those at 21% MC and 24 degrees C were statistically significant (alpha <= 0.05). Incubation conditions and time of prior storage had a noticeable yet relatively minor effect on which fungi infected the kernels.
机译：使用二氧化碳（CO2）测试试剂盒评估了带壳玉米对贮藏真菌侵袭的敏感性。将去壳的玉米样品再湿润至水分含量（MC）为16％或21％wb，并在密封的玻璃罐中于24或30摄氏度下孵育<= 80小时。使用指示剂凝胶的色号（0-5）对广口瓶中的CO2浓度进行定量，该色号是通过与色卡（VR）进行目视比较或使用Digital Color Reader（DCR）确定的。 DCR颜色数字与时间的关系曲线比VR颜色数字的关系更为平滑。 DCR能够更好地区分样品之间的差异，尤其是当色数小于2.0时。色数对麦角固醇差异的线性回归的最高确定系数（r（2））对于DCR读数为0.7，对于VR读数为0.73。在所有四种温度-MC组合下，真菌敏感性的排名都相似，并且将其他条件下的读数与21％MC和24摄氏度下的读数进行线性回归的许多r（2）值具有统计学意义（alpha <= 0.05）。孵育条件和事先保存的时间对真菌感染谷粒的影响是明显的，但影响相对较小。

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