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首页> 外文期刊>Cellular Signalling >A role for phosphoinositides in tyrosine phosphorylation of p125 focal adhesion kinase in rat pancreatic acini
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A role for phosphoinositides in tyrosine phosphorylation of p125 focal adhesion kinase in rat pancreatic acini

机译：磷酸肌醇在大鼠胰腺腺泡中p125黏着斑激酶酪氨酸磷酸化中的作用

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Previous studies have shown that different agonists increase tyrosine phosphorylation of the focal adhesion related proteins p125(FAK), p130(Cas), and paxillin in different cell types and that tyrosine phosphorylation depends on the integrity of the actin cytoskeleton. Because phosphoinositides are important for the maintenance of the cytoskeleton, the role of phosphoinositides in the tyrosine phosphorylation of these proteins in response to occupancy of m3 muscarinic and CCKA receptors has been investigated in pancreatic acini. Addition of carbachol or CCK-8 to pancreatic acini resulted in rapid increases in the tyrosine phosphorylation of p125(FAK), p130(Cas), and paxillin. Pretreatment of pancreatic acini with LY294002 or wortmannin resulted in a concentration-dependent inhibition of tyrosine phosphorylation of p125(FAK), p130(Cas), and paxillin stimulated by carbachol or CCK-8. Carbachol- or CCK-8-stimulated tyrosine phosphorylation of these proteins was not inhibited by rapamycin, PD 98059 or SE 203580, and thus it was dissociated from the activation of p70 S6 or MAP kinases. These results indicate that m3 muscarinic and CCKA receptor-mediated increase in p125(FAK) , p130(Cas), and paxillin tyrosine phosphorylation in pancreatic acini depends on the ability of these cells to synthesise phosphoinositides. (C) 2000 Elsevier Science inc. All rights reserved. [References: 58]
机译：先前的研究表明，在不同的细胞类型中，不同的激动剂会增加粘着相关蛋白p125（FAK），p130（Cas）和paxillin的酪氨酸磷酸化，酪氨酸的磷酸化取决于肌动蛋白细胞骨架的完整性。由于磷酸肌醇对于维持细胞骨架非常重要，因此已在胰腺腺泡中研究了磷酸肌醇在响应于m3毒蕈碱和CCKA受体的占用而酪氨酸磷酸化这些蛋白中的作用。向胰腺腺泡中添加卡巴胆碱或CCK-8会导致p125（FAK），p130（Cas）和paxillin的酪氨酸磷酸化迅速增加。用LY294002或渥曼青霉素对胰腺腺泡进行预处理可导致浓度受到抑制，其抑制了卡巴胆碱或CCK-8刺激的p125（FAK），p130（Cas）和paxillin酪氨酸磷酸化。雷帕霉素，PD 98059或SE 203580不能抑制这些蛋白的卡巴胆碱或CCK-8刺激的酪氨酸磷酸化，因此，它与p70 S6或MAP激酶的激活无关。这些结果表明m3毒蕈碱和CCKA受体介导的胰腺腺泡蛋白p125（FAK），p130（Cas）和paxillin酪氨酸磷酸化增加取决于这些细胞合成磷酸肌醇的能力。 （C）2000爱思唯尔科学公司版权所有。 [参考：58]

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