...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Signalling >Reactive oxygen species and Wnt signalling crosstalk patterns mouse extraembryonic endoderm
【24h】

Reactive oxygen species and Wnt signalling crosstalk patterns mouse extraembryonic endoderm

机译：活性氧和Wnt信号串扰模式的小鼠胚内胚层

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Primitive endoderm formation from the inner cell mass is one of the earliest known cell fate decisions made in the mouse embryo. The mechanisms involved in orchestrating this process are not fully understood and are difficult to study in vivo. The F9 teratocarcinoma cell line is an in vitro model used to circumvent many technical problems surrounding the study of extraembryonic endoderm differentiation. F9 cells treated with retinoic acid differentiate to primitive endoderm and this is accompanied by the activation of canonical Wnt-β-catenin signalling. Reactive oxygen species can modulate this signalling pathway[1], but whether they are sufficient to induce extraembryonic endoderm in vitro is not known. In the present study, a sustained increase in ROS levels was found in retinoic acid-treated F9 cells. An increase in Tcf-Lef transcriptional activity, a read out of Wnt-β-catenin signalling, was also seen in response to exogenous H _2O _2. Analysis from immunoblots, immunocytochemistry and real time PCR revealed the presence of markers of differentiation and a reduction in the expression of a marker of proliferation, confirming that H _2O _2-treated F9 cells developed into primitive endoderm. In contrast, exposing retinoic acid-treated cells to antioxidants impeded differentiation. Real time PCR was also used to identify candidates responsible for the observed elevation in ROS production. Results indicated that the NADPH oxidase 1, 2, 3 and 4 and Duox2 genes were RA responsive. Furthermore, the NADPH oxidase inhibitor, diphenyleneiodonium chloride was shown to attenuate primitive endoderm formation. Together, these results shed new light on how early mouse embryogenesis might be influenced by the crosstalk involving ROS and the Wnt-β-catenin signalling pathway.
机译：由内部细胞团形成的原始内胚层形成是小鼠胚胎中做出的最早的已知细胞命运决定之一。编排此过程涉及的机制尚未完全了解，并且很难在体内进行研究。 F9畸胎瘤细胞系是一种体外模型，用于规避围绕胚外内胚层分化研究的许多技术问题。用视黄酸处理的F9细胞分化为原始内胚层，并伴有经典Wnt-β-catenin信号的激活。活性氧可以调节这种信号通路[1]，但是尚不清楚它们是否足以在体外诱导胚外内胚层。在本研究中，在视黄酸处理的F9细胞中发现ROS水平持续升高。还响应外源H _2O _2，从Wnt-β-catenin信号中读出了Tcf-Lef转录活性的增加。通过免疫印迹，免疫细胞化学和实时PCR进行的分析显示，存在分化标记，并且增殖标记的表达减少，这证实了H _2O _2处理的F9细胞已发育成原始内胚层。相反，将视黄酸处理过的细胞暴露于抗氧化剂会阻碍分化。实时PCR还用于鉴定引起ROS产生中观察到的升高的候选物。结果表明NADPH氧化酶1、2、3和4和Duox2基因对RA有反应。此外，NADPH氧化酶抑制剂二苯二碘鎓氯化物可减弱原始内胚层的形成。在一起，这些结果为涉及ROS和Wnt-β-catenin信号通路的串扰如何影响早期小鼠胚胎发生提供了新的思路。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号