A novel calmodulin-β-PIX interaction and its implication in receptor tyrosine kinase regulation

Singh V.K.; Munro K.; Jia Z.

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A novel calmodulin-β-PIX interaction and its implication in receptor tyrosine kinase regulation

机译：钙调蛋白-β-PIX的新型相互作用及其在受体酪氨酸激酶调节中的意义

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Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates numerous cellular processes, primarily in response to calcium flux. We have identified and characterized a novel interaction between CaM and β-p21-activated kinase interacting exchange factor (β-PIX), a putative guanine exchange factor implicated in cell signaling, using affinity pull-down assays, co-immunoprecipitation, co-localization and circular dichroism studies. Fluorescence-based titration and isothermal titration calorimetry experiments revealed a Ca ~(2+)-dependent binding mechanism (K _D≤10μM). Further, we show that CaM participates in a multi-protein complex involving β-PIX and E3 ubiquitin ligase c-Cbl (casitas B-cell lymphoma), which may play a critical role in receptor tyrosine kinase regulation and downstream signaling.

机译：钙调蛋白（CaM）是一种普遍存在的钙结合蛋白，主要是响应钙通量来调节许多细胞过程。我们已经使用亲和力下拉测定法，共免疫沉淀法，共定位法确定了CaM与β-p21激活的激酶相互作用交换因子（β-PIX）之间的新型相互作用，β-PIX是一种牵涉在细胞信号传导中的鸟嘌呤交换因子。和圆二色性研究。基于荧光的滴定和等温滴定量热法实验揭示了Ca〜（2+）依赖性的结合机理（K_D≤10μM）。此外，我们显示CaM参与了涉及β-PIX和E3泛素连接酶c-Cbl（卡西塔斯B细胞淋巴瘤）的多蛋白复合物，这可能在受体酪氨酸激酶调节和下游信号传导中起关键作用。

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《Cellular Signalling》 |2012年第9期|共7页
作者
Singh V.K.; Munro K.; Jia Z.;
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正文语种 eng
中图分类细胞形态学;
关键词
β-PIX; Calcium binding; Calmodulin; Cell signaling; Protein-protein interaction; Receptor tyrosine kinase;

机译：β-PIX;钙结合;钙调蛋白;细胞信号转导;蛋白-蛋白质相互作用;受体酪氨酸激酶;

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专利

1. A novel calmodulin-β-PIX interaction and its implication in receptor tyrosine kinase regulation [J] . Singh V.K., Munro K., Jia Z. Cellular Signalling . 2012,第9期

机译：钙调蛋白-β-PIX的新型相互作用及其在受体酪氨酸激酶调节中的意义
2. Structural Basis of Allosteric Ligand-Receptor Interactions in the Insulin/Relaxin Peptide Family: Implications for Other Receptor Tyrosine Kinases and G-Protein-Coupled Receptors [J] . Pierre De Meyts, Lisbeth Gauguin, Angela Manegold Svendsen, Annals of the New York Academy of Sciences . 2009,第期

机译：胰岛素/松弛素肽家族中的变构配体-受体相互作用的结构基础：对其他受体酪氨酸激酶和G-蛋白偶联受体的影响。
3. Inhibition of tyrosine kinase activity of the epidermal growth factor (EGF) receptor by a truncated receptor form that binds to EGF: role for interreceptor interaction in kinase regulation. [J] . A Basu, M Raghunath, S Bishayee, Molecular and Cellular Biology . 1989,第2期

机译：结合EGF的截短受体形式对表皮生长因子（EGF）受体酪氨酸激酶活性的抑制：受体间相互作用在激酶调节中的作用。
4. Carboxyl Group Footprinting MS Provides Structural Insights into Inter- and Intramolecular Interactions of the Oncogenic HER2-HER3 Receptor Tyrosine Kinase Heterodimer [C] . Timothy S. Collier, John D. Monsey, Ron Bose American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：羧基脚印MS为致癌癌癌患者的间晶酶激酶异二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二草的结构见解提供结构洞察力
5. Activation of the receptor tyrosine kinase p185(neu) is dependent on dimerization via specific interreceptor interactions [D] . Burke, Christine Lynn 1997

机译：受体酪氨酸激酶p185（neu）的激活取决于特定受体间相互作用的二聚作用
6. Inhibition of tyrosine kinase activity of the epidermal growth factor (EGF) receptor by a truncated receptor form that binds to EGF: role for interreceptor interaction in kinase regulation. [O] . A Basu, M Raghunath, S Bishayee, 1989

机译：结合EGF的截短受体形式抑制表皮生长因子（EGF）受体的酪氨酸激酶活性：受体间相互作用在激酶调节中的作用。
7. Inhibition of tyrosine kinase activity of the epidermal growth factor (EGF) receptor by a truncated receptor form that binds to EGF: role for interreceptor interaction in kinase regulation. [O] . Basu, A, Raghunath, M, Bishayee, S, 1989

机译：结合EGF的截短受体形式抑制表皮生长因子（EGF）受体的酪氨酸激酶活性：受体间相互作用在激酶调节中的作用。
8. Egf Receptor Mabs and Chemotherapy/Characterization of Synergistic Interactions Between Cyrotoxic Agents and Inhibitors of the Tyrosine Kinase Growth Factor Receptor Signaling Cascade [R] . Seidman, A. 1999

机译：Egf受体单克隆抗体和化学疗法/ Cyrotoxic剂与酪氨酸激酶生长因子受体信号级联抑制剂之间协同相互作用的表征

A novel calmodulin-β-PIX interaction and its implication in receptor tyrosine kinase regulation

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