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首页> 外文期刊>Cellular & molecular biology letters. >Endothelial microparticle formation in moderate concentrations of homocysteine and methionine in vitro.
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Endothelial microparticle formation in moderate concentrations of homocysteine and methionine in vitro.

机译：在中等浓度的同型半胱氨酸和蛋氨酸中体外形成内皮微粒。

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Microparticles (MPs) are small membrane vesicles released by stimulated or apoptotic cells, including the endothelium. Hyperhomocysteinemia (HHcy) is a blood disorder characterized by an increase in the plasma concentrations of total homocysteine (Hcy). The plasma Hcy level is determined by environmental factors (dietary habits, i.e. the intake of folic acid, FA) and genetic factors (N (5),N (10)-methylenetetrahydro-folate reductase, MTHFR, polymorphism 677C>T). To evaluate whether moderate Hcy concentrations induce endothelial MP formation, the role of FA supplementation and the influence of MTHFR polymorphism were analysed. Human umbilical vein endothelial cells (HUVEC) were treated in vitro with 50 muM of Hcy and methionine (Met). The MP number and apoptotic phenotype were analyzed using flow cytometry. Increasing doses of FA (5, 15 and 50 muM) were used to reduce the HHcy effect. The MTHFR 677C>T polymorphism was determined. HUVEC stimulated by Hcy produced significantly more MPs than HUVEC under the control conditions: 3,551 +/- 620 vs 2,270 +/- 657 kMP (p = 0.02). Supplementation with FA at concentrations of 5, 15 and 50 muM reduced the MP count in the cell culture supernatant to 345 +/- 332, 873 +/- 329, and 688 +/- 453 kMP, respectively (p = 0.03). MTHFR 677C>T heterozygosity was associated with a significant increase in MP formation after stimulation with Hcy compared to the control conditions: 3,617 +/- 152 vs 1,518 +/- 343 kMP (p = 0.02). Furthermore, the MTHFR genotype altered MP formation after Met loading. On average, 24% of the entire MP population was apoptotic (annexin V-positive). Endothelial function impairment due to HHcy is related to MP shedding, which may involve platelets and other blood and vascular cells. MP shedding is a physiological response to moderate HHcy.
机译：微粒（MPs）是受刺激或凋亡的细胞（包括内皮细胞）释放的小膜囊泡。高同型半胱氨酸血症（HHcy）是一种血液疾病，其特征在于总同型半胱氨酸（Hcy）的血浆浓度增加。血浆Hcy水平取决于环境因素（饮食习惯，即叶酸的摄入量，FA）和遗传因素（N（5），N（10）-亚甲基四氢叶酸还原酶，MTHFR，多态性677C> T）。为了评估中等浓度的Hcy是否诱导内皮细胞MP的形成，分析了FA的补充作用和MTHFR多态性的影响。用50μM的Hcy和蛋氨酸（Met）体外处理人脐静脉内皮细胞（HUVEC）。使用流式细胞仪分析MP值和凋亡表型。使用增加剂量的FA（5、15和50μM）来降低HHcy效应。确定了MTHFR 677C> T多态性。在对照条件下，Hcy刺激的HUVEC产生的MP明显多于HUVEC：3551 +/- 620 vs 2270 +/- 657 kMP（p = 0.02）。添加浓度为5、15和50μM的FA分别将细胞培养上清液中的MP计数分别降低至345 +/- 332、873 +/- 329和688 +/- 453 kMP（p = 0.03）。与对照条件相比，用Hcy刺激后，MTHFR 677C> T杂合性与MP形成显着增加有关：3,617 +/- 152 vs 1,518 +/- 343 kMP（p = 0.02）。此外，MTHFR基因型改变了Met加载后MP的形成。平均而言，整个MP人口中有24％是凋亡的（annexin V阳性）。 HHcy引起的内皮功能损害与MP脱落有关，MP脱落可能涉及血小板以及其他血液和血管细胞。 MP脱落是对中度HHcy的生理反应。

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