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首页> 外文期刊>Cellular & molecular biology letters. >Thy28 partially prevents apoptosis induction following engagement of membrane immunoglobulin in WEHI-231 B lymphoma cells.
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Thy28 partially prevents apoptosis induction following engagement of membrane immunoglobulin in WEHI-231 B lymphoma cells.

机译：Thy28部分阻止膜免疫球蛋白与WEHI-231 B淋巴瘤细胞结合后的凋亡诱导。

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摘要

Thy28 protein is conserved among plants, bacteria, and mammalian cells. Nuclear Thy28 protein is substantially expressed in testis, liver, and immune cells such as lymphocytes. Lymphocyte apoptosis plays a crucial role in homeostasis and formation of a diverse lymphocyte repertoire. In this study, we examined whether Thy28 affects induction of apoptosis in WEHI-231 B lymphoma cells following engagement of membrane immunoglobulin (mIg). Once they were established, the Thy28-overexpressing WEHI-231 cells showed similar expression levels of IgM and class I major histocompatibility complex (MHC) molecule compared with controls. The Thy28-overexpressing cells were considerably resistant to loss of mitochondrial membrane potential (ΔΨm), caspase-3 activation, and increase in annexin-positive cells upon mIg engagement. These changes were concomitant with an increase in G1 phase associated with upregulation of p27(Kip1). The anti-IgM-induced sustained activation of c-Jun N-terminal kinase (JNK), which was associated with late-phase hydrogen peroxide (H(2)O(2)) production, was partially reduced in the Thy28-expressing cells relative to controls. Taken together, the data suggest that in WEHI-231 B lymphoma cells, Thy28 regulates mIg-mediated apoptotic events through the JNK-H(2)O(2) activation pathway, concomitant with an accumulation of cells in G1 phase associated with upregulation of p27(Kip1) in WEHI-231 B lymphoma cells.
机译：Thy28蛋白在植物，细菌和哺乳动物细胞中保守。核Thy28蛋白在睾丸，肝脏和免疫细胞（如淋巴细胞）中大量表达。淋巴细胞凋亡在体内平衡和多样化淋巴细胞库的形成中起着至关重要的作用。在这项研究中，我们检查了Thy28是否影响膜免疫球蛋白（mIg）参与后WEHI-231 B淋巴瘤细胞的凋亡诱导。一旦建立，与对照相比，过量表达Thy28的WEHI-231细胞显示出相似的IgM和I类主要组织相容性复合物（MHC）分子表达水平。过量表达Thy28的细胞对线粒体膜电位（ΔΨm）的丧失，半胱天冬酶3激活和mIg参与后膜联蛋白阳性细胞的增加具有相当大的抵抗力。这些变化与p27（Kip1）上调相关的G1期增加有关。抗IgM诱导的c-Jun N末端激酶（JNK）的持续活化，与后期过氧化氢（H（2）O（2））生产相关，在表达Thy28的细胞中部分减少相对于控件。综上所述，数据表明，在WEHI-231 B淋巴瘤细胞中，Thy28通过JNK-H（2）O（2）激活途径调节mIg介导的凋亡事件，并伴随G1期上调与细胞上调相关。 WEHI-231 B淋巴瘤细胞中的p27（Kip1）。

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