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Regulation of phospholipase D by P2X(7) receptors in submandibular ductal cells

机译：下颌下腺导管细胞中P2X（7）受体对磷脂酶D的调节

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ATP (1 mM) increased the phospholipase D (PLD) activity of rat submandibular gland (RSMG) ductal cells in a concentration-dependent and calcium-sensitive manner. The response to ATP was reproduced by benzoylbenzoyl-ATP (Bz-ATP, 100 muM) and also partly by adenosine 5'-(gamma-thio)triphosphate (ATPgammaS, 1 mM). A similar stimulation was observed in control mice (P2X(7)R(+/+) mice) but not in mice lacking the P2X(7) receptors (P2X(7)R(-/-) mice). Oxidized ATP and Coomassie blue or the addition of magnesium or nickel to the incubation medium inhibited the response to ATP. The stimulation of PLD by purinergic agonist was inhibited by about 50% by calphostin C and chelerythrine, two protein kinase C (PKC) inhibitors. The stimulation of PLD by Bz-ATP and by o-tetradecanoylphorbol 13-acetate (TPA), a phorbol ester which activates PKC, were not additive. From these results we can conclude that the activation of P2X(7) receptors in RSMG ductal cells is coupled to the activation of a PLD. This activation is partly mediated by protein kinase C. (C) 2003 Elsevier Science Inc. All rights reserved. [References: 54]
机译：ATP（1 mM）以浓度依赖性和钙敏感的方式增加了大鼠下颌腺（RSMG）导管细胞的磷脂酶D（PLD）活性。对ATP的反应通过苯甲酰基苯甲酰-ATP（Bz-ATP，100μM）再现，还部分通过腺苷5'-（γ-硫代）三磷酸（ATPgammaS，1 mM）再现。在对照小鼠（P2X（7）R（+ / +）小鼠）中观察到类似的刺激，但在缺少P2X（7）受体的小鼠（P2X（7）R（-/-）小鼠）中观察不到。氧化的ATP和考马斯亮蓝或向培养液中添加镁或镍会抑制对ATP的反应。嘌呤能C和白屈菜红碱是两种蛋白激酶C（PKC）抑制剂，嘌呤能激动剂对PLD的刺激作用被抑制了约50％。 Bz-ATP和邻十四碳酰佛波醇13-乙酸酯（TPA）（一种激活PKC的佛波酯）对PLD的刺激作用不是加性的。从这些结果，我们可以得出结论，RSMG导管细胞中P2X（7）受体的激活与PLD的激活相关。这种激活部分地由蛋白激酶C介导。（C）2003 Elsevier Science Inc.保留所有权利。 [参考：54]

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