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首页> 外文期刊>Cellular & molecular biology letters. >MECHANICAL STRAIN AFFECTS SOME microRNA PROFILES IN PRE-OETEOBLASTS
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MECHANICAL STRAIN AFFECTS SOME microRNA PROFILES IN PRE-OETEOBLASTS

机译：机械应变影响前成骨细胞中的某些microRNA谱。

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摘要

MicroRNAs (miRNAs) are important regulators of cell proliferation, differentiation and function. Mechanical strain is an essential factor for osteoblast proliferation and differentiation. A previous study revealed that a physiological mechanical tensile strain of 2500 microstrain (mu epsilon) at 0.5 Hz applied once a day for 1 h over 3 consecutive days promoted osteoblast differentiation. However, the mechanoresponsive miRNAs of these osteoblasts were not identified. In this study, we applied the same mechanical tensile strain to in vitro cultivated mouse MC3T3-E1 pre-osteoblasts and identified the mechanoresponsive miRNAs. Using miRNA microarray and qRT-PCR assays, the expression patterns of miRNAs were evaluated and 5 of them were found to be significantly different between the mechanical loading group and the control group: miR-3077-5p, 3090-5p and 3103-5p were significantly upregulated and miR-466i-3p and 466h-3p were downregulated. Bioinformatics analysis revealed possible target genes for these differentially expressed miRNAs. Some target genes correlated with osteoblast differentiation. These findings indicated that the mechanical strain changed the expression levels of these miRNAs. This might be a potential regulator of osteoblast differentiation and responses to mechanical strain.
机译：微小RNA（miRNA）是细胞增殖，分化和功能的重要调节剂。机械应变是成骨细胞增殖和分化的重要因素。先前的一项研究表明，连续3天每天一次连续1天施加0.5 h的2500微应变（μepsilon）的生理机械拉伸应变可促进成骨细胞的分化。但是，这些成骨细胞的机械反应性miRNA尚未确定。在这项研究中，我们将相同的机械拉伸应变应用于体外培养的小鼠MC3T3-E1前成骨细胞，并鉴定了机械响应性miRNA。使用miRNA芯片和qRT-PCR分析，评估了miRNA的表达模式，发现其中5个在机械负荷组和对照组之间存在显着差异：miR-3077-5p，3090-5p和3103-5p为明显上调，而miR-466i-3p和466h-3p下调。生物信息学分析揭示了这些差异表达的miRNA的可能靶基因。一些靶基因与成骨细胞分化相关。这些发现表明机械应变改变了这些miRNA的表达水平。这可能是成骨细胞分化和对机械应变反应的潜在调节剂。

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