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首页> 外文期刊>Cellular & molecular biology letters. >ZNF300, a recently identified human transcription factor, activates the human IL-2Rbeta promoter through the overlapping ZNF300/EGR1 binding site.
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ZNF300, a recently identified human transcription factor, activates the human IL-2Rbeta promoter through the overlapping ZNF300/EGR1 binding site.

机译：ZNF300是最近发现的人类转录因子，它通过重叠的ZNF300 / EGR1结合位点激活人类IL-2Rbeta启动子。

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摘要

ZNF300 was recently identified as a member of the human KRAB/C(2)H(2) zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5'-GCGGGGGCG-3' of Egr1, another member of the KRAB/C(2)H(2) zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5'-CTGGGGGCG-3'. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rbeta promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rbeta promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rbeta promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rbeta promoter.
机译：ZNF300最近被确定为人类KRAB / C（2）H（2）锌指蛋白家族的成员。 ZNF300在人类基因调控网络中的作用鲜为人知。在这项研究中，进一步分析了ZNF300的DNA结合特性。我们发现重组ZNF300可以结合Egr1，KRAB / C（2）H（2）锌指蛋白家族的另一个成员的结合位点5'-GCGGGGGCG-3'。同样，重组Egr1也显示出与ZNF300结合位点5'-CTGGGGGCG-3'相似的结合亲和力。生物信息学分析表明，人IL-2Rbeta启动子区域中存在重叠的ZNF300 / Egr1结合位点，先前已知该位点可被内源性Egr1识别。电泳迁移率变动分析表明，内源性ZNF300也可以结合该位点。瞬时转染测定表明ZNF300和Egr1均可激活IL-2Rbeta启动子，并且激活因重叠ZNF300 / Egr1结合位点中残基的突变而被取消。 ZNF300和Egr1的共表达导致增强的IL-2Rbeta启动子活性。因此，ZNF300可能是人类IL-2Rbeta启动子的另一个调节子。

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