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首页> 外文期刊>Cellular & molecular biology letters. >A small sequence in domain v of the mitochondrial large ribosomal RNA restores Drosophila melanogaster pole cell determination in uv-irradiated embryos
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A small sequence in domain v of the mitochondrial large ribosomal RNA restores Drosophila melanogaster pole cell determination in uv-irradiated embryos

机译：线粒体大核糖体RNA的结构域v中的一个小序列恢复了紫外线照射胚胎中果蝇的极细胞测定。

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摘要

The mechanism by which the mitochondrial large rRNA is involved in the restoration of the pole cell-forming ability in Drosophila embryos is still unknown. We identified a 15-ribonucleotide sequence which is conserved from the protobacterium Wolbachia to the higher eukaryotes in domain V of the mitochondrial large rRNA. This short sequence is sufficient to restore pole cell determination in UV-irradiated Drosophila embryos. Here, we provide evidence that the conserved 15-base sequence is sufficient to restore luciferase activity in vitro. Moreover, we show that the internal GAGA sequence is involved in protein binding and that mutations in this tetranucleotide affect the sequence's ability to restore luciferase activity. The obtained results lead us to propose that mtlrRNA may be involved either in damaged protein reactivation or in protein biosynthesis during pole cell determination.
机译：线粒体大rRNA参与果蝇胚胎中极细胞形成能力的恢复的机制仍是未知的。我们确定了从原细菌Wolbachia保守到线粒体大rRNA域V中较高的真核生物的15个核糖核苷酸序列。该短序列足以恢复紫外线照射的果蝇胚胎中的极细胞测定。在这里，我们提供的证据表明，保守的15个碱基的序列足以在体外恢复荧光素酶的活性。此外，我们表明内部GAGA序列参与蛋白质结合，并且该四核苷酸中的突变影响序列恢复荧光素酶活性的能力。获得的结果使我们提出，mtlrRNA可能参与极细胞测定过程中受损的蛋白质重新活化或蛋白质的生物合成。

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