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首页> 外文期刊>Cellular Signalling >Regulation of membrane-bound PKC in adult cardiac ventricular myocytes
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Regulation of membrane-bound PKC in adult cardiac ventricular myocytes

机译：膜结合PKC在成年心脏室肌细胞中的调节

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Activation of protein kinase C (PKC) is thought to involve translocation to the particulate fraction. The present study demonstrates a membrane-associated, inactive pool of PKC in adult rat ventricular myocytes. Membranes were isolated from stimulated (phorbol 12-myristate 13-acetate (PMA), endothelin-1 (ET-1)) or control myocytes and PKC activity determined in the absence (active PKC) or presence (total PKC) of PMA. An inactive, PMA-responsive, pool of PKC was detected. In intact myocytes, PMA or ET-1 induced a translocation of PKCepsilon from the cytosol into the particulate fraction. In contrast, ET-1 decreased both total and active PKC in the membranes: this decrease was associated with a loss of PKCepsilon immunoreactivity. PMA increased the amount of membrane-associated, inactive PKC. Our results demonstrate the presence of a membrane-associated pool of PKC in cardiac myocytes that is differentially modulated by ET-1 or PMA. (C) 2003 Elsevier Science Inc. All rights reserved. [References: 43]
机译：认为蛋白激酶C（PKC）的激活涉及易位至颗粒级分。本研究表明成年大鼠心室肌细胞中膜相关，PKC的非活动池。从刺激的（佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA），内皮素-1（ET-1））或对照心肌细胞中分离膜，并在不存在PMA（活性PKC）或存在PMA（总PKC）的情况下确定PKC活性。检测到无效的PMA响应性PKC库。在完整的心肌细胞中，PMA或ET-1诱导PKCepsilon从胞质溶胶转移到颗粒级分中。相反，ET-1降低了膜中的总PKC和活性PKC：这种下降与PKCepsilon免疫反应性降低有关。 PMA增加了与膜相关的非活性PKC的数量。我们的结果表明，心肌细胞中存在与PKC相关的膜相关池，该池由ET-1或PMA差异调节。 （C）2003 Elsevier Science Inc.保留所有权利。 [参考：43]

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