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首页> 外文期刊>Oncology letters >Epithelial-mesenchymal transition mediates anoikis resistance and enhances invasion in pleural effusion-derived human lung cancer cells
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Epithelial-mesenchymal transition mediates anoikis resistance and enhances invasion in pleural effusion-derived human lung cancer cells

机译：上皮-间充质转化介导的厌食症抵抗并增强了胸腔积液来源的人肺癌细胞的侵袭

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摘要

Epithelial-mesenchymal transition (EMT) is implicated in cancer pathological processes, particularly cancer invasion and metastasis. The present study demonstrated that EMT was critical for the metastasic potential of lung cancer cells isolated from a patient. P1 primary lung cancer cells were found to exhibit increased anoikis resistance compared with established A549, H23 and H460 lung cancer cells. Results of migration and invasion assays revealed that the invasion capability of P1 and A549 cells was higher than that of H23 and H460 cells. However, the migration of P1 cells was similar to that of H23 and H460 cells while A549 demonstrated a superior migrating ability. Western blot analysis indicated that while E-cadherin levels in all lung cancer cells were identified as comparable, P1 cells expressed the highest levels of N-cadherin. In the present study, detachment of cells was demonstrated for the first time to stimulate further transition of E-cadherin to N-cadherin. In addition, this obervation was more pronounced in P1 cells. These observations highlight the importance of EMT in cancer metastasis. In order to study the effect of ethnicity on cancer cell behavior, in the future a large number of Thai patient-derived cell lines must be analyzed.
机译：上皮-间质转化（EMT）与癌症病理过程有关，特别是癌症的侵袭和转移。本研究表明，EMT对从患者体内分离出的肺癌细胞的转移潜力至关重要。与已建立的A549，H23和H460肺癌细胞相比，发现P1原发性肺癌细胞显示出更高的厌氧耐药性。迁移和侵袭实验的结果表明，P1和A549细胞的侵袭能力高于H23和H460细胞。然而，P1细胞的迁移与H23和H460细胞的迁移相似，而A549表现出了优异的迁移能力。蛋白质印迹分析表明，虽然所有肺癌细胞中的E-钙粘蛋白水平都可以确定，但P1细胞表达的N-钙粘蛋白水平最高。在本研究中，第一次证明了细胞的脱离，以刺激E-钙粘蛋白进一步转变为N-钙粘蛋白。另外，这种观察在P1细胞中更为明显。这些观察结果强调了EMT在癌症转移中的重要性。为了研究种族对癌细胞行为的影响，将来必须分析大量泰国患者来源的细胞系。

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