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首页> 外文期刊>Oncology letters >Mesenchymal stem cells derived from breast cancer tissue promote the proliferation and migration of the MCF-7 cell line in vitro
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Mesenchymal stem cells derived from breast cancer tissue promote the proliferation and migration of the MCF-7 cell line in vitro

机译：源自乳腺癌组织的间充质干细胞在体外促进MCF-7细胞系的增殖和迁移

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摘要

Mesenchymal stem cells (MSCs) are critical in promoting cancer progression, including tumor growth and metastasis. MSCs, as a subpopulation of cells found in the tumor microenvironment, have been isolated from several tumor tissues, but have not been isolated from breast cancer tissue to date. Therefore, the purpose of this study was to isolate MSCs from primary human breast cancer tissue, and to study the effect of breast cancer MSCs (BC-MSCs) on the proliferation and migration of the MCF-7 cell line in vitro. MSCs were isolated and identified from primary breast cancer tissue obtained from 9 patients. The MCF-7 cell line was treated with 10 and 20% breast cancer-associated MSC (BC-MSC)-conditioned medium (CM) for 10-48 h, and changes in proliferation and migration were observed. Furthermore, we investigated the migration of 10 and 20% CM concentrations on MCF-7 through a scratch wound assay and a transwell migration assay. We successfully isolated and identified MSCs from primary breast cancer tissues. BC-MSCs showed characteristics similar to those of bone marrow MSCs, and possessed the capability of multipotential differentiation into osteoblasts and adipocytes. The results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that 10 and 20% CM concentrations increased the proliferation of MCF-7 cells to different levels. The results also revealed a greater increase in different levels compared with the control group. In conclusion, MSCs were confirmed to exist in human breast cancer tissues, and BC-MSCs may promote the proliferation and migration of breast cancer cells.
机译：间充质干细胞（MSC）在促进癌症进展（包括肿瘤生长和转移）中至关重要。 MSC是在肿瘤微环境中发现的细胞亚群，已从数个肿瘤组织中分离出来，但迄今为止尚未从乳腺癌组织中分离出来。因此，本研究的目的是从原发性人类乳腺癌组织中分离MSC，并研究乳腺癌MSC（BC-MSC）对体外MCF-7细胞增殖和迁移的影响。从9名患者获得的原发性乳腺癌组织中分离并鉴定了MSC。 MCF-7细胞系用10％和20％乳腺癌相关的MSC（BC-MSC）条件培养基（CM）处理10-48 h，观察到增殖和迁移的变化。此外，我们通过刮擦伤口测定和transwell迁移测定研究了MCF-7上10％和20％CM浓度的迁移。我们成功地从原发性乳腺癌组织中分离并鉴定了MSC。 BC-MSCs具有与骨髓MSCs相似的特征，并具有多能分化为成骨细胞和脂肪细胞的能力。 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑鎓溴化物（MTT）分析的结果表明，浓度为10％和20％的CM将MCF-7细胞的增殖提高至不同水平。结果还显示与对照组相比，不同水平的更大增加。总之，证实了MSCs存在于人乳腺癌组织中，并且BC-MSCs可以促进乳腺癌细胞的增殖和迁移。

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