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首页> 外文期刊>Oncology letters >Clinical implications of Girdin and PI3K protein expression in breast cancer
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Clinical implications of Girdin and PI3K protein expression in breast cancer

机译：Girdin和PI3K蛋白表达在乳腺癌中的临床意义

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The aim of this study was to investigate the correlation between Girdin and PI3K in breast cancer stem cells and the clinical implications of the co-expression of these two proteins in breast cancer patients. CD44(+)/CD24(-) tumor cells from the MD-231 cell line were sorted by flow cytometry. The expression status of Girdin and PI3K proteins was detected using western blotting and immunohistochemical staining. The relationship between Girdin and PI3K proteins and clinicopathological parameters was analyzed in 820 breast cancer patients. Girdin and PI3K proteins were more highly expressed in CD44(+)/CD24(-) tumor stem cells compared to the control group and Girdin and PI3K proteins were co-immunoprecipitated in the MD-231 cell line. Of the 820 enrolled breast cancer patients, Girdin and PI3K proteins were expressed in 295 (35.98%) and 492 (60.00%) cases, respectively. There were 162 (19.76%) cases which co-expressed Girdin and PI3K proteins. Univariate and multivariate analyses indicated that the co-expression of Girdin and PI3K proteins correlated with histological type, metastatic nodes and distant metastasis (P=0.01, 0.001 and 0.001, respectively). After analyzing survival rates, cases with Girdin and PI3K co-expression were shown to attain a significantly increased distant metastasis rate and poorer postoperative, disease-specific survival compared to those with Girdin and PI3K co-expression (P=0.001). In the Cox regression test, Girdin and PI3K co-expression was detected as an independent prognostic factor (P=0.001). Girdin may regulate the biological behavior of breast cancer via the PI3K/Akt/mTOR pathway, and thus, serve as a potential new target for breast cancer treatment.
机译：这项研究的目的是调查乳腺癌干细胞中Girdin和PI3K之间的相关性以及这两种蛋白在乳腺癌患者中共表达的临床意义。通过流式细胞术对来自MD-231细胞系的CD44（+）/ CD24（-）肿瘤细胞进行分类。使用免疫印迹和免疫组化染色检测Girdin和PI3K蛋白的表达状态。分析了820名乳腺癌患者中Girdin和PI3K蛋白与临床病理参数之间的关系。与对照组相比，Girdin和PI3K蛋白在CD44（+）/ CD24（-）肿瘤干细胞中的表达更高，而Girdin和PI3K蛋白则在MD-231细胞系中共同免疫沉淀。在820名登记的乳腺癌患者中，Girdin和PI3K蛋白分别在295（35.98％）和492（60.00％）例中表达。共表达Girdin和PI3K蛋白的162例（19.76％）。单因素和多因素分析表明，Girdin和PI3K蛋白的共表达与组织学类型，转移淋巴结和远处转移相关（分别为P = 0.01、0.001和0.001）。在分析了存活率之后，与Girdin和PI3K共表达的患者相比，Girdin和PI3K共表达的患者显示远处转移率显着提高，且术后疾病特异性生存率较差（P = 0.001）。在Cox回归测试中，检测到Girdin和PI3K共表达是独立的预后因素（P = 0.001）。 Girdin可能通过PI3K / Akt / mTOR途径调节乳腺癌的生物学行为，因此，可能成为乳腺癌治疗的新靶标。

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