...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Oncology reports >Silencing MARCH1 suppresses proliferation, migration and invasion of ovarian cancer SKOV3 cells via downregulation of NF-kappa B and Wnt/beta-catenin pathways
【24h】

Silencing MARCH1 suppresses proliferation, migration and invasion of ovarian cancer SKOV3 cells via downregulation of NF-kappa B and Wnt/beta-catenin pathways

机译：沉默MARCH1通过下调NF-κB和Wnt /β-catenin途径抑制卵巢癌SKOV3细胞的增殖，迁移和侵袭

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Membrane-associated RING-CH (MARCH) belongs to the family of RING-CH type E3 ubiquitin ligases. MARCH1 ubiquitinates and downregulates MHC class II expression in APCs and targets major players of the immune system. However, the role of MARCH1 in ovarian cancer has not been elucidated. The present study investigated the function of MARCH1 in ovarian cancer and the potential mechanisms involved. MARCH1 expression was examined in human ovarian cancer tissue specimens by immunohistochemistry. The role of MARCH1 in ovarian cancer cells was assessed by cell proliferation, migration and invasion assays with MARCH1 gene silencing. To investigate the mechanism by which MARCH1 functions, correlation between MARCH1 and the cell signaling pathways were analyzed using a luciferase reporter assay, real-time RT-PCR, western blot assay and immunofluorescence. MARCH1 was found to be over-expressed in ovarian cancer tissues when compared to adjacent non-tumor and normal ovarian tissues. Silencing MARCH1 inhibited SKOV3 cell proliferation, invasion and migration, as well as inhibiting the NF-kappa B and the Wnt/beta-catenin pathways. MARCH1 functions as a tumor promoter by upregulating the NF-kappa B and the Wnt/beta-catenin pathways, indicating that MARCH1 may be a therapeutic target for patients with ovarian cancer.
机译：膜相关的RING-CH（MARCH）属于RING-CH型E3泛素连接酶家族。 MARCH1泛素化并下调APC中II类MHC的表达，并靶向免疫系统的主要参与者。但是，尚未阐明MARCH1在卵巢癌中的作用。本研究调查了MARCH1在卵巢癌中的功能及其潜在的机制。通过免疫组织化学检查了人卵巢癌组织标本中的MARCH1表达。通过MARCH1基因沉默的细胞增殖，迁移和侵袭试验评估了MARCH1在卵巢癌细胞中的作用。为了研究MARCH1发挥作用的机制，使用荧光素酶报告基因测定，实时RT-PCR，蛋白质印迹测定和免疫荧光分析了MARCH1与细胞信号通路之间的相关性。与相邻的非肿瘤组织和正常卵巢组织相比，发现MARCH1在卵巢癌组织中过表达。沉默MARCH1抑制SKOV3细胞增殖，侵袭和迁移，并抑制NF-κB和Wnt /β-catenin途径。 MARCH1通过上调NF-κB和Wnt /β-catenin途径作为肿瘤启动子，表明MARCH1可能是卵巢癌患者的治疗靶标。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号