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首页> 外文期刊>Biological chemistry >Mutual Activation of Two Restriction Endonucleases: Interaction of EcoP1 and EcoP15
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Mutual Activation of Two Restriction Endonucleases: Interaction of EcoP1 and EcoP15

机译：两种限制性内切核酸酶的相互激活：EcoP1和EcoP15的相互作用

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摘要

Type III restriction endonucleases recognize non symmetric nucleotide sequences. A necessary condition for DNA cleavage is the presence of two unmethylated recognition sites which are inversely ('head-to-head') oriented in the DNA double strand. A DNA substrate possessing one EcoP1 and one EcoP15 site in the head-to-head configuration could not be cleaved by the individual enzymes, however, it was specifically digested in the simultaneous presence of both enzymes. In agreement with the tracking collision model for the DNA interaction of type III enzymes cleavage could be abolished by Lac repressor bound between the two sites. We conclude that two different type III enzymes can functionally cooperate in the cleavage of DNA.
机译：III型限制性核酸内切酶可识别非对称核苷酸序列。 DNA切割的必要条件是存在两个未甲基化的识别位点，它们在DNA双链中反向（“头对头”）定向。在头对头构型中具有一个EcoP1和一个EcoP15位点的DNA底物不能被单个酶裂解，但是，在同时存在两种酶的情况下，它被特异性地消化。与跟踪碰撞模型相一致，III型酶的DNA相互作用可通过两个位点之间的Lac阻遏物结合而消除。我们得出的结论是，两种不同的III型酶可以在DNA切割中发挥功能。

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