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首页> 外文期刊>Research in Microbiology >Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu).
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Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu).

机译：中国浅湖（东湖）富营养化和贫营养化盆地中氨氧化细菌的多样性和丰富性。

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摘要

Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH(+)(4)) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status.
机译：基于氨单加氧酶基因（amoA）的经典栽培和分子方法被用于研究湖泊沉积物中β-变形杆菌氨氧化细菌（AOB）的丰度和多样性。对中国浅湖（东湖）的富营养化和贫营养化盆地进行了氨（NH（+）（4））浓度的采样。在富营养化盆地中，AOB数显着较低，而在富营养化盆地中，AOB数显着较高。此外，利用针对amoA的限制性片段长度多态性，在富营养化盆地中发现了十种限制性模式，包括六个独特的模式，而在富营养性盆地中发现了五种模式，只有一个独特的限制性群。对AOB的系统发育分析表明，与亚硝基亚硝化单胞菌和脲亚硝化单胞菌相关的AOB和与亚硝基螺菌相关的AOB普遍存在。前者在富营养化盆地中占主导地位（87.2％），而后者则在富营养化盆地中占主导地位（65.5％）。此外，仅在富营养化盆地中检出了与亚硝化单胞菌相关的AOB，比例很小（3.2％）。这些结果表明，在营养状态不同的湖泊中，沉积物AOB的选择和适应性强。

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