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首页> 外文期刊>Research in Microbiology >Molecular identification of the first human isolate belonging to the Veillonella ratti-Veillonella criceti group based on 16S rDNA and dnaK gene sequencing.
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Molecular identification of the first human isolate belonging to the Veillonella ratti-Veillonella criceti group based on 16S rDNA and dnaK gene sequencing.

机译:基于16S rDNA和dnaK基因测序的第一批人类分离株属于Veillonella ratti-Veillonella criceti组。

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摘要

Anaerobic Gram-negative cocci belonging to the genus Veillonella are currently subdivided into eight species. Among them, four have been isolated either from human flora or from clinical samples during infectious processes: Veillonella atypica, V. dispar, V. parvula and V. montpellierensis. To date, the four other species have only been found in animals. In particular, V. ratti and V. criceti, since their characterization, have been exclusively reported from mouth and intestine of rodents. We report here for the first time a human isolate belonging to the V. ratti-V. criceti group recovered in mixed aerobic-anaerobic flora from a semen sample in a 24-year-old man attending the urology unit of our hospital for infertility. Identification of the isolate was based on 16S ribosomal DNA and dnaK gene sequencing. Since then, two strains of Veillonella sp. recovered from semen samples remained unidentified to the species level, and 16S rDNA-based phylogenetic analysis revealed that they might represent novel taxa within the genus Veillonella. Taken together, these observations suggest that host restriction may not exist in the genus Veillonella and that bacterial diversity remains underestimated both within this genus and in human semen.
机译:Veillonella属的厌氧革兰氏阴性球菌目前细分为八种。其中,从人类菌群或在感染过程中从临床样品中分离出四种:非典型Veillonella atypica,V。dispar,V。parvula和V. montpellierensis。迄今为止,仅在动物中发现了其他四个物种。特别地,自啮齿动物起,V。ratti和V. criceti被专门从啮齿动物的口腔和肠中报告。我们在这里首次报告属于鼠弧菌V的人分离株。 criceti组从一名24岁男子的精液样本中恢复到需氧-厌氧混合菌群,该男子正在我们医院不育症泌尿科就诊。分离物的鉴定基于16S核糖体DNA和dnaK基因测序。从那时起,两株Veillonella sp。从精液样品中回收的菌种仍未鉴定到物种水平,基于16S rDNA的系统发育分析表明,它们可能代表Veillonella属中的新分类群。综上所述,这些观察结果表明,Veillonella属中可能不存在宿主限制,并且该属和人类精液中的细菌多样性仍然被低估了。

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