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首页> 外文期刊>Ophthalmic plastic and reconstructive surgery >Myotoxic effects of the skeletal muscle-specific immunotoxin, ricin-mAb35, on orbicularis oculi muscle after eyelid injections in rabbits.
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Myotoxic effects of the skeletal muscle-specific immunotoxin, ricin-mAb35, on orbicularis oculi muscle after eyelid injections in rabbits.

机译：兔眼睑注射后，骨骼肌特异性免疫毒素蓖麻毒素-mAb35对眼球菌的肌毒性作用。

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PURPOSE: The authors recently demonstrated that a single injection of the immunotoxin ricin-mAb35 has potent and long-lasting myotoxic effects in extraocular muscles. The myotoxicity of injected ricin-mAb35 was tested in the eyelids of rabbits to determine its potential for use in the treatment of benign essential blepharospasm and other dystonias. METHODS: The immunotoxin ricin-mAb35 was injected in one eyelid of adult rabbits. After 1 week, 1 month, or 6 months, the rabbits were euthanized, and the eyelids were prepared for histologic examination of inflammatory cell infiltrate with immunohistochemical localization of cd11b and myosin heavy chain isoform expression. Muscle loss was quantified by analysis of muscle fiber cross-sectional area and total myofiber number. RESULTS: Within the first week after a single injection of ricin-mAb35, some edema developed, which resolved by the second week. Otherwise, the eyelids were normal in appearance. A short-lived inflammatory response was seen at 1 week, but this resolved 1 month after treatment. One week after injection, there was a significant decrease in the total number of orbicularis oculi myofibers in the ricin-mAb35-treated eyelids. This myofiber loss remained significant 1 month later and was maintained 6 months after the initial injection. CONCLUSIONS: Direct injection of the immunotoxin ricin-mAb35 resulted in significant, acute muscle loss in the orbicularis oculi of rabbits that was maintained for up to 6 months. Physiologic studies are needed to demonstrate concomitant loss of muscle strength, but these results suggest that ricin-mAb35 injection holds promise as a muscle-weakening agent in the eyelid.
机译：目的：作者最近证明，单次注射免疫毒素蓖麻毒素-蓖麻毒素-mAb35对眼外肌具有强效和持久的肌毒性作用。在家兔的眼睑中测试了注射蓖麻毒素-mAb35的肌毒性，以确定其在治疗良性原发性眼睑痉挛和其他肌张力障碍中的潜力。方法：将免疫毒素蓖麻毒蛋白-mAb35注射到成年兔的一只眼睑中。 1周，1个月或6个月后，对兔子实施安乐死，并准备眼睑，用cd11b的免疫组织化学定位和肌球蛋白重链亚型表达对炎性细胞浸润进行组织学检查。通过分析肌纤维横截面积和总肌纤维数量来量化肌损失。结果：单次注射蓖麻毒素-mAb35后的第一个星期内，出现了一些水肿，第二个星期就消失了。否则，眼睑外观正常。在1周时发现了短暂的炎症反应，但是在治疗后1个月就消失了。注射后1周，经蓖麻毒素-mAb35处理的眼睑中眼球菌的肌纤维总数明显减少。肌纤维损失在1个月后仍然显着，并在初次注射后6个月得以维持。结论：直接注射免疫毒素蓖麻毒蛋白-mAb35导致兔子的眼球眼严重急性肌肉丢失，并维持长达6个月。需要进行生理学研究来证明肌肉力量的同时丧失，但这些结果表明，蓖麻毒素-mAb35注射液有望在眼睑中使肌肉减弱。

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