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Generation of a hollow dark spherical spot by 4pi focusing of a radially polarized Laguerre-Gaussian beam

机译:通过径向极化的拉盖尔-高斯光束的4pi聚焦产生空心的黑色球形斑点

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摘要

The properties of the focal spot for 4pi focusing with radially polarized first-order Laguerre-Gaussian beams are calculated. It is shown that a focal spot that has an extremely sharp dark region at the center and an almost-perfect spherical symmetry can be achieved. When such a hollow dark spherical spot is used in 4pi fluorescence depletion microscopy, an axial FWHM spot size of approx39 nm and a transverse FWHM spot size of approx64 nm can be achieved simultaneously in a practical system.
机译:计算了用径向偏振的一阶拉盖尔-高斯光束对4pi聚焦的焦点的特性。结果表明,可以实现在中心具有非常尖锐的暗区和几乎完美的球对称性的焦点。当在4pi荧光耗尽显微镜中使用这种空心暗球形斑点时,在实际系统中可以同时实现大约39 nm的轴向FWHM点尺寸和大约64 nm的横向FWHM点尺寸。

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