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A modular culture system for the generation of multiple specialized tissues.

机译：用于生成多种特殊组织的模块化培养系统。

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Numerous factors influence cell functions and tissue development in culture. A modular culture system has been developed to allow the control of many of these important environmental parameters. Optimal adhesion of cells is obtained by selecting an individual biomaterial. Selected specimens are mounted in a tissue carrier in order to protect it against damage during handling and after seeding cells, the carriers can be used in a series of compatible perfusion culture containers. This technique allows the simple bathing of growing tissue under continuous medium transport and the exposure of epithelia to a gradient with different fluids at the luminal and basal sides. A further container is made of transparent material to observe microscopically the developing tissue. In addition, a special model features a flexible silicone lid to apply force to mimic the mechanical load required for developing connective and muscular tissue. Perfusion culture of stem/progenitor cells at the interface of an artificial interstitium made by a polyester fleece results in the spatial development of tubules. During long term culture over weeks the growing tissue is continuously exposed to fresh nutrition and respiratory gas. The medium is transported in a constant flow or in pulses, preventing unstirred layers of fluid. A variety of applications of this modular system, described in this paper, demonstrates that the biological profile of cells and tissues can be strongly improved when perfusion culture with a permanent provision of fresh medium is applied.
机译：许多因素影响培养中的细胞功能和组织发育。已经开发了模块化培养系统以允许控制许多这些重要的环境参数。通过选择单个生物材料可获得最佳的细胞粘附。选定的标本安装在组织载体中，以保护其在处理过程中和接种细胞后不受损坏，这些载体可用于一系列兼容的灌注培养容器中。该技术可以在连续的介质传输下简单地对生长中的组织进行沐浴，并使上皮在管腔和基底侧的不同流体梯度下暴露。另一个容器由透明材料制成，以便在显微镜下观察发育中的组织。此外，特殊型号还具有一个可弯曲的硅胶盖，可施加力来模仿结缔和肌肉组织发育所需的机械负荷。在由聚酯纤维制成的人造间质的界面上进行干/祖细胞的灌注培养会导致肾小管的空间发育。在数周的长期培养过程中，生长中的组织不断暴露于新鲜的营养和呼吸气体中。介质以恒定的流量或脉冲形式传输，从而防止了未搅拌的流体层。本文描述的此模块化系统的各种应用表明，当使用永久性新鲜培养基进行灌注培养时，可以大大改善细胞和组织的生物学特性。

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