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首页> 外文期刊>Optics Letters >Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium
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Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

机译：双波长数字全息显微镜同时进行细胞形态测定和折射率测量，以及灌注介质的染料增强分散

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摘要

Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelength DHM that allows us to separately measure both the RI and the cellular thickness by exploiting an enhanced dispersion of the perfusion medium achieved by the utilization of an extracellular dye. The two wavelengths are chosen in the vicinity of the absorption peak of the dye, where the absorption is accompanied by a significant variation of the RI as a function of the wavelength.
机译：数字全息显微镜（DHM）可以实现纳米精度的光程差（OPD）测量。透明细胞诱导的OPD取决于细胞的折射率（RI）及其形态。这封信提出了一种双波长DHM，使我们能够通过利用利用细胞外染料实现的灌注介质增强分散来分别测量RI和细胞厚度。在染料的吸收峰附近选择两个波长，其中吸收伴随着RI随波长的显着变化。

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