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Single-beam homodyne SPIDER for multiphoton microscopy

机译:用于多光子显微镜的单光束零差SPIDER

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摘要

We report a new version of spectral phase interferometry for direct electric field reconstruction (SPIDER) requiring only a single phase-shaped laser beam. A narrowband probe pulse is selected out of a broadband ultrafast laser pulse by a phase pulse-shaping technique and mixed with the original broadband pulse to generate a second-harmonic generation (SHG) signal. Using another SHG signal solely generated by the broadband pulse as a local oscillator, the spectral phase of the broadband laser pulse can be analytically retrieved by a combination of double-quadrature spectral interferometry and homodyne optical technique for SPIDER (HOT SPIDER). An arbitrary spectral phase at the sample position of a microscope can be compensated with a precision of 0.05 rad over the FWHM of the laser spectrum. It is readily applicable to a nonlinear microscopy technique with a phase-controlled broadband laser pulse.
机译:我们报告了仅需单相形激光束的直接电场重建(SPIDER)光谱相位干涉术的新版本。通过相位脉冲整形技术从宽带超快激光脉冲中选择一个窄带探测脉冲,并将其与原始宽带脉冲混合以产生二次谐波(SHG)信号。使用仅由宽带脉冲产生的另一个SHG信号作为本地振荡器,可以通过双正交光谱干涉测量法和用于SPIDER(HOT SPIDER)的零差光学技术的组合来解析地检索宽带激光脉冲的光谱相位。显微镜样品位置上的任意光谱相位可以在激光光谱的FWHM上以0.05 rad的精度进行补偿。它很容易应用于具有相控宽带激光脉冲的非线性显微镜技术。

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