Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination

Kengyeh K. Chu; Daryl Lim; Jerome Mertz

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Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination

机译：自适应照明增强了双光子显微镜中的弱信号灵敏度

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We describe a technique to enhance both the weak-signal relative sensitivity and the dynamic range of alaser scanning optical microscope. The technique is based on maintaining a fixed detection power by fast feedback control of the illumination power, thereby transferring high measurement resolution to weak signals while virtually eliminating the possibility of image saturation. We analyze and demonstrate the benefits of adaptive illumination in two-photon fluorescence microscopy.

机译：我们描述了一种技术，可同时提高弱信号相对灵敏度和激光扫描光学显微镜的动态范围。该技术基于通过对照明功率的快速反馈控制来维持固定的检测功率，从而将高测量分辨率转换为微弱信号，同时实际上消除了图像饱和的可能性。我们分析并证明了在双光子荧光显微镜中自适应照明的好处。

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《Optics Letters》 |2007年第19期|共3页
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Kengyeh K. Chu; Daryl Lim; Jerome Mertz;
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正文语种 eng
中图分类光学;
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专利

1. Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination [J] . Kengyeh K. Chu, Daryl Lim, Jerome Mertz Optics Letters . 2007,第19期

机译：自适应照明增强了双光子显微镜中的弱信号灵敏度
2. Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination [J] . Keisuke Isobe, Takanori Takeda, Kyohei Mochizuki, Biomedical Optics Express . 2013,第11期

机译：通过将时间聚焦与结构化照明相结合来提高双光子荧光显微镜的横向分辨率和光学切片能力
3. Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination [J] . Keisuke Isobe, Takanori Takeda, Kyohei Mochizuki, Biomedical Optics Express . 2013,第11期

机译：通过将时间聚焦与结构化照明相结合来提高双光子荧光显微镜的横向分辨率和光学切片能力
4. Fast Two-Snapshot Structured Illumination for Wide-Field Two-Photon Microscopy with Enhanced Axial Resolution and Signal-to-Noise Ratio [C] . Yunlong Meng, Wei Lin, Jialong Chen, Conference on Lasers and Electro-Optics . 2019

机译：具有增强的轴向分辨率和信噪比的宽视野双光子显微镜快速双快照结构照明
5. Multiscale Imaging of the Mouse Cortex Using Two-Photon Microscopy and Wide-Field Illumination [D] . Bumstead, Jonathan R. 2017

机译：使用双光子显微镜和宽场照明的小鼠皮质的多尺度成像
6. Nanodiamonds enable adaptive-optics enhanced super-resolution two-photon excitation microscopy [O] . Graeme E. Johnstone, Gemma S. Cairns, Brian R. Patton 2019

机译：纳米金刚石可实现自适应光学增强的超分辨率双光子激发显微镜
7. Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination [O] . Kengyeh K. Chu, Daryl Lim, Jerome Mertz 2007

机译：自适应照明增强了双光子显微镜中的弱信号灵敏度

Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination

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