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En face imaging of single cell layers by differential phase-contrast optical coherence microscopy

机译:通过差分相差光学相干显微镜对单细胞层进行全脸成像

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摘要

Optical coherence microscopy (OCM) is capable of imaging the backscattering potential of a sample with high transversal and axial resolution. We report on a combination of OCM with a differential phase-contrast technique that permits imaging of the subwavelength optical path differences that occur between a narrow beam probing a sample and its surrounding. This technique allows small transversal refractive-index variations close to a selected interface to be seen. We report on the method and present first images of a test sample and a single cell layer. The cells act as phase objects; imaging the phase properties improves the contrast compared with that of intensity images.
机译:光学相干显微镜(OCM)能够以高的横向和轴向分辨率成像样品的反向散射电位。我们报告了OCM与微分相位对比技术的组合,该技术允许对在探测样品的窄光束及其周围环境之间发生的亚波长光程差进行成像。该技术允许看到接近所选界面的较小的横向折射率变化。我们报告了该方法,并介绍了测试样品和单个细胞层的第一张图像。细胞充当相位对象。与强度图像相比,对相位属性进行成像可以改善对比度。

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