We present a novel sampling imaging technique capable of performing simultaneous two-dimensional measurements of the temporal and spectral characteristics of light-emission processes by use of a specially designed streak camera. A proof-of-principle experiment was performed with a homemade multifocal multiphoton fluorescence microscope. The system was calibrated with a Fabry-Perot etalon and a standard fluorophore solution (rhodamine 6G in ethanol) and was shown to have temporal and spectral resolution of 6.5 ps and 3 nm, respectively, as well as high accuracy and reproducibility in lifetime and spectrum measurement. Temporally and spectrally resolved images of 4 X 4 foci on the sample can be obtained with a snapshot.
展开▼
机译：我们提出了一种新颖的采样成像技术，该技术能够通过使用专门设计的条纹相机对发光过程的时间和光谱特征进行同时的二维测量。用自制的多焦点多光子荧光显微镜进行了原理验证实验。该系统已使用Fabry-Perot标准具和标准荧光团溶液（若丹明6G在乙醇中）进行了校准，显示的时间和光谱分辨率分别为6.5 ps和3 nm，并且在寿命和光谱方面具有较高的准确性和可重复性测量。可以通过快照获得样品上4 X 4焦点的临时和光谱分辨图像。
展开▼
