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Spatial photoselection of single molecules on the surface of spherical microcavities

机译:球形微腔表面上单分子的空间光选择

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We show that ultrasensitive microdroplet-stream fluorescence techniques combined with surfactant forms of Rhodamine dyes can be used to probe single molecules on the surfaces of spherical microcavities. Individual octadecyl Rhodamine B molecules, shown previously by ensemble measurements to be localized and oriented at the surfaces of liquid microspheres, were spatially photoselected primarily along great circles lying perpendicular or parallel to the detection axis by use of polarized laser excitation. A polarization dependence is observed in the distribution of single-molecule fluorescence amplitudes that can be interpreted qualitatively in terms of position-dependent fluorescence-collection efficiencies. (C) 1998 Optical Society of America. [References: 18]
机译:我们显示超灵敏的微滴流荧光技术与若丹明染料的表面活性剂形式相结合可用于探测球形微腔表面上的单个分子。总体上通过集合测量显示的单个十八烷基若丹明B分子在液体微球表面上定位并定向,主要是通过使用偏振激光激发沿沿垂直于或平行于检测轴的大圆进行空间光选择。在单分子荧光振幅的分布中观察到偏振依赖性,这可以根据位置依赖性荧光收集效率定性地解释。 (C)1998年美国眼镜学会。 [参考:18]

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