Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

G. Valentini; C. DAndrea; D. Comelli

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Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

机译：通过增强的CCD进行时间分辨的DNA微阵列读取以实现最终灵敏度

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We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.

机译：我们描述了一种基于时间分辨荧光测量的DNA芯片读取的新技术。我们使用具有皮秒分辨率的增强型CCD相机从标有花青3的突变DNA微阵列中获取了一组延时荧光图像。我们分别测量了标记和背景的荧光寿命，并使用此信息计算了从延时图像开始，标记的振幅图。该程序使我们能够识别在连续波检测中获得的荧光图像中不可见的杂交斑点。

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《Optics Letters》 |2000年第22期|共3页
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G. Valentini; C. DAndrea; D. Comelli;
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正文语种 eng
中图分类光学;
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1. Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity [J] . G. Valentini, C. DAndrea, D. Comelli Optics Letters . 2000,第22期

机译：通过增强的CCD进行时间分辨的DNA微阵列读取以实现最终灵敏度
2. Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging [J] . Mitchell AC., Wall JE., Murray JG., Journal of Microscopy . 2002,第Pta3期

机译：直接调制CCD检测器的有效灵敏度：时间分辨荧光成像的新方法
3. Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging. [J] . Mitchell AC, Wall JE, Murray JG, Journal of Microscopy . 2002,第Pta3期

机译：直接调制CCD检测器的有效灵敏度：时间分辨荧光成像的新方法。
4. Time resolved reading of DNA microarray by an intensified CCD camera [C] . R. Cubeddu, C. DAndrea, A. Pifferi, Conference on Lasers and Electro-Optics Europe . 2000

机译：通过强化CCD相机解决了DNA微阵列的时间
5. Fluorescence-enhanced optical imaging on three-dimensional phantoms using a hand-held probe based frequency-domain intensified charge coupled device (ICCD) optical imager. [D] . Ge, Jiajia. 2008

机译：使用基于手持式探头的频域增强电荷耦合器件（ICCD）光学成像仪对三维体模进行荧光增强的光学成像。
6. Progress in electron-multiplying CCD (EMCCD) based high-resolution high-sensitivity x-ray detector for fluoroscopy and radiography [O] . Andrew T. Kuhls, Girijesh Yadava, Vikas Patel, -1

机译：用于荧光检查和射线照相的基于电子倍增CCD（EMCCD）的高分辨率高灵敏度X射线探测器的进展
7. Beam induced fluorescence monitor development: Comparison of image intensified CCD and electron multiplying CCD cameras [O] . Shutko Y., Hoffmann D. H. H., Forck P., 2015

机译：光束诱导荧光监视器的发展：图像增强型CCD和电子倍增CCD相机的比较

Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

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