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Sub-100 nm IR spectromicroscopy of living cells

机译:活细胞的亚100 nm红外光谱

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We have performed IR spectromicroscopy of cells immersed in liquid water, with a lateral resolution better than 100 nm. Here, we use the motion of an atomic force microscope tip, probing the local transient deformation induced by an IR pulsed laser tuned at a sample absorbing wavelength. By Fourier analysis of the vibration of the cantilever tip, we can discriminate frequencies that are characteristic of the object, thus eliminating the influence of the water absorption. This opens the door of chemical imaging of living species in vivo, with spatial resolution of the order of the size of cell components.
机译:我们对浸入液态水中的细胞进行了红外光谱分析,其横向分辨率优于100 nm。在这里,我们使用原子力显微镜尖端的运动,探测由在样品吸收波长处调谐的红外脉冲激光引起的局部瞬态变形。通过对悬臂尖端的振动进行傅立叶分析,我们可以区分物体的特征频率,从而消除吸水的影响。这开启了活体生物化学成像的大门,其空间分辨率达到了细胞成分大小的数量级。

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