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Time-wavelength two-dimensional femtosecond fluorescence imaging

机译:时间波长二维飞秒荧光成像

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摘要

We report a new femtosecond time-resolved fluorescence spectrometer that enables us to observe fluorescence intensity as a time-wavelength two-dimensional image in a single measurement. This method utilizes a time-to-space conversion technique and fluorescence sum-frequency mixing with a femtosecond gate pulse. It provides a fluorescence image covering temporal and spectral spans of ~2 ps and ~60 nm, respectively. Calibration of the time and intensity axes of the image is made by use of a long-lived dye fluorescence. The two-dimensional fluorescence image of β-carotene obtained demonstrates the high potential of this method for quantitative studies of ultrafast excited-state dynamics.
机译:我们报告了一种新的飞秒时间分辨荧光光谱仪,它使我们能够在一次测量中将荧光强度观察为时间波长二维图像。该方法利用了时空转换技术以及与飞秒选通脉冲的荧光和频混合。它提供的荧光图像分别覆盖了约2 ps和约60 nm的时间和光谱范围。通过使用长寿命的染料荧光来校准图像的时间轴和强度轴。所获得的β-胡萝卜素的二维荧光图像证明了该方法在超快激发态动力学定量研究中的巨大潜力。

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