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首页> 外文期刊>Optics Letters >Total internal reflection ellipsometry: ultrahigh sensitivity for protein adsorption on metal surfaces
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Total internal reflection ellipsometry: ultrahigh sensitivity for protein adsorption on metal surfaces

机译:全内反射椭圆光度法:对蛋白质在金属表面的吸附具有超高灵敏度

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摘要

Total internal reflection ellipsometry (TIRE) is used to study adsorption of human serum albumin and fibrinogen on thin gold films. TIRE shows very high sensitivity for protein monolayers adsorbed on metal surfaces when surface plasmon resonance effects are utilized. The measured data, expressed in ellipsometric angles psi and DELTA are of several orders of magnitude larger in comparison with those from similar experiments performed with traditional ellipsometry. TIRE in spectral mode opens a new path for precise studies of organic layers adsorbed on metal surfaces, with a potential for resolving the adsorbed layer microstructure.
机译:全内反射椭圆光度法(TIRE)用于研究人血清白蛋白和纤维蛋白原在金薄膜上的吸附。当利用表面等离振子共振效应时,轮胎对吸附在金属表面的蛋白质单分子膜显示出很高的敏感性。与椭圆偏振法的类似实验相比,以椭圆角psi和DELTA表示的测量数据要大几个数量级。光谱模式的轮胎为精确研究金属表面吸附的有机层开辟了一条新途径,并有可能解决吸附层的微观结构。

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