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Portable in situ fluorescence cytometry of microscale cell-based assays

机译:基于微型细胞的便携式原位荧光细胞分析

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摘要

A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog ((mu)CCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a (mu)CCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the (mu)CCA devices daunorubicin accumulation is proportional to the liquid turnover rate.
机译:已经开发了便携式荧光细胞计数系统,用于表征微型细胞培养类似物(μCCA)设备中的化学浓度和细胞状态。该系统基于分立的光学组件,提供了适用于各种基于细胞的微量测定的实时图像测量的模块化平台。作为一项可行性研究,我们调查了在(μ)CCA隔室中培养的小鼠L细胞摄取柔红霉素的实时动态。通过便携式荧光细胞计数系统测量的时间过程结果证实,在(μ)CCA设备中,柔红霉素的积累与液体周转率成正比。

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