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首页> 外文期刊>Optics Letters >Multiphoton polarization and generalized polarization microscopy reveal oleic-acid-induced structural changes in intercellular lipid layers of the skin
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Multiphoton polarization and generalized polarization microscopy reveal oleic-acid-induced structural changes in intercellular lipid layers of the skin

机译:多光子极化和广义极化显微镜揭示油酸诱导的皮肤细胞间脂质层的结构变化

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摘要

We have demonstrated that both multiphoton polarization and generalized polarization (GP) microscopy may be combined to characterize the structural changes of intercellular lipids in skin. Both polarization and GP (at 440- and 490-nm emission) images obtained by analysis of Laurdan fluorescence suggest that the treatment of oleic acid results in a skin surface with a more random packing of lipid molecules, which allows easier water penetration. Our results show that combined polarization and GP microscopy can be used to characterize the physical and chemical changes in biological structures. (C) 2004 Optical Society of America.
机译:我们已经证明多光子极化和广义极化(GP)显微镜可以结合起来表征皮肤中细胞间脂质的结构变化。通过Laurdan荧光分析获得的偏振图像和GP图像(在440和490 nm发射下)均表明,油酸处理会导致皮肤表面脂质分子的堆积更加随机,从而使水更易于渗透。我们的结果表明,偏振和GP显微镜相结合可用于表征生物结构的物理和化学变化。 (C)2004年美国眼镜学会。

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