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In vivo fluorescence microscopy of neuronal activity in three dimensions by use of voltage-sensitive dyes

机译:使用电压敏感染料的三维神经元活性体内荧光显微镜检查

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We report in vivo imaging of neuronal electrical activity from superficial layers of the mouse barrel cortex. The measurements have ~16-μm spatial and 3-ms temporal resolution and reach depths of 150 μm below the cortical surface. The depth-dependent differential-fluorescence optical sections of activity are consistent with known cortical architecture and represent an important step toward in vivo measurement of functioning complex neural networks. Our observations employ a custom gradient-index lens probe and voltage-sensitive dye fluorescence; the use of epi-illumination rather than dark-field illumination provides the dramatic signal-to-noise improvement necessary for fast three-dimensional imaging.
机译:我们报告从小鼠桶皮层的表层神经元电活动的体内成像。这些测量具有〜16μm的空间分辨率和3 ms的时间分辨率,并在皮质表面以下达到了150μm的深度。活动的与深度有关的差分荧光光学部分与已知的皮质结构一致,并且代表了朝着体内测量功能复杂的神经网络迈出的重要一步。我们的观察结果使用了定制的梯度折射率透镜探针和电压敏感型染料荧光。使用落射照明而不是暗视场照明可以显着提高快速三维成像所需的信噪比。

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