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首页> 外文期刊>Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics >Detection of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens in chronic endodontic infection.
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Detection of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens in chronic endodontic infection.

机译：在慢性牙髓感染中检测牙龈卟啉单胞菌，牙龈卟啉单胞菌，中间型小球藻和黑头球菌。

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摘要

Black-pigmented anaerobic rods such as Prevotella spp. and Porphyromonas spp. are involved in the etiology and perpetuation of endodontic infections. The aim of this study was to evaluate the prevalence of these species in chronic endodontic infections by using culture and polymerase chain reaction (PCR) techniques. Samples of 100 patients with root canals displaying chronic endodontic infections were obtained by sterilized paper points. Bacterial identification was performed by using culture and PCR techniques. By culture, in 33% of the samples, P. intermedia-P. nigrescens (75.8%), P. gingivalis (27.3%), and P. endodontalis (9.1%) were identified, and by PCR 60% of the samples harbored P. nigrescens (43.3%), P. gingivalis (43.3%), P. intermedia (31.7%), and P. endodontalis (23.3%). The presence of these black-pigmented anaerobic rods alone or in association in chronic endodontic infections seems to be frequent. PCR is a very sensitive technique for detecting DNA from bacterial cells. Culturing is onlyable to reveal living bacteria and is less sensitive for the identification of low numbers of bacterial cells.
机译：黑色色素厌氧棒，如Prevotella spp。和卟啉单胞菌属。参与牙髓感染的病因和永续性。这项研究的目的是通过使用培养和聚合酶链反应（PCR）技术评估这些物种在慢性牙髓感染中的患病率。通过灭菌纸点获得100例表现出慢性牙髓感染的根管患者的样本。通过使用培养和PCR技术进行细菌鉴定。通过培养，在33％的样品中，中间P.-P。 nigrescens（75.8％），P。gingivalis（27.3％）和endotaltalis（9.1％）被鉴定，通过PCR的样本中有60％携带了Nigrescens（43.3％），P。gingivalis（43.3％），中间假单胞菌（31.7％）和牙髓假单胞菌（23.3％）。这些黑色色素厌氧棒单独存在或与慢性牙髓感染一起存在似乎很常见。 PCR是检测细菌细胞DNA的非常灵敏的技术。培养仅能揭示活细菌，并且对于识别少量细菌细胞不那么敏感。

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