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首页> 外文期刊>Oral oncology >p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers.
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p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers.

机译：吸烟者和非吸烟者正常口腔黏膜原代培养物中的p53突变和蛋白质表达。

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摘要

The normal oral mucosa from 77 individuals of known smoking and alcohol-intake history was cultured. After 14-21 days in culture, epithelial cells were stained for p53 expression using immunohistochemistry. PCR-SSCP analysis of the p53 gene was also performed on a random sub-set of these samples (20 non-smokers and 21 smokers). Expression of the stable, non-functional form of p53 protein as detected by the p53-240 antibody was found to be significantly elevated in the cultured oral mucosa of smokers. (P < 0.01). PCR-SSCP analyses indicated a higher level of base changes in smokers than in non-smokers. These observations are consistent with other findings of significantly increased p53 protein expression in the oral mucosa and other tissues of smokers and suggests that p53 mutations may be an early event in smoking-induced oral cancers.
机译：培养了77名已知吸烟和饮酒史的人的正常口腔粘膜。培养14-21天后，使用免疫组织化学对上皮细胞进行p53表达染色。还对这些样品的随机亚组（20位非吸烟者和21位吸烟者）进行了p53基因的PCR-SSCP分析。通过p53-240抗体检测到的稳定的，非功能性形式的p53蛋白的表达在吸烟者的口腔粘膜培养物中明显升高。 （P <0.01）。 PCR-SSCP分析表明，吸烟者的碱基变化水平高于不吸烟者。这些观察结果与在吸烟者的口腔粘膜和其他组织中p53蛋白表达显着增加的其他发现一致，并表明p53突变可能是吸烟引起的口腔癌的早期事件。

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