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首页> 外文期刊>Oral oncology >hMLH1 promoter methylation is an early event in oral cancer.
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hMLH1 promoter methylation is an early event in oral cancer.

机译：hMLH1启动子甲基化是口腔癌的早期事件。

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Promoter methylation is believed to inactivate the expression of hMLH1. This process has been implicated in the tumorigenesis of oral squamous cell carcinoma (OSCC). Thus, the aim of this study was to determine the profile of hMLH1 methylation and protein expression in OSCC. The matched case-control study included 50 OSCC cases and 200 controls, with a median of age 64 (Q-Q 54-71) years. Protein expression was determined by immunohistochemical staining, and hMLH1 gene promoter methylation was analyzed by methylation-specific polymerase chain reaction (MSP). A conditional logistic regression model for risk factors was built for OSCC cases and matched controls. Promoter methylation of hMLH1 was detected in 38 (76%) OSCC cases, but in none of the control samples. Of the 38 OSCC samples with promoter methylation, 12 (32%) were negative for hMLH1 protein, and corresponded to early clinical stages (10 in stage II and 2 in stage I). All 12 unmethylated samples showed positive stain for hMLH1. Multiple logistic regression analysis showed an OR of 16.54 (IC 95%: 1.69-161.68, p=0.016) for methylation of the hMLH1 gene and early stages of OSCC, adjusting by gender and tobacco use. This study showed a high frequency of hMLH1 promoter methylation that occurred in most of the early stage cases and in about half of the late stage cases. It is proposed that hMLH1 promoter methylation is an early event that is maintained during tumor progression.
机译：据信启动子甲基化使hMLH1的表达失活。该过程与口腔鳞状细胞癌（OSCC）的肿瘤发生有关。因此，本研究的目的是确定OSCC中hMLH1甲基化和蛋白质表达的概况。匹配的病例对照研究包括50例OSCC病例和200例对照，中位年龄为64岁（Q-Q 54-71）。通过免疫组织化学染色确定蛋白质表达，并通过甲基化特异性聚合酶链反应（MSP）分析hMLH1基因启动子的甲基化。针对OSCC病例和匹配的对照建立了风险因素的条件逻辑回归模型。在38（76％）OSCC病例中检测到了hMLH1的启动子甲基化，但没有对照样品。在38个具有启动子甲基化的OSCC样本中，有12个（32％）的hMLH1蛋白阴性，对应于早期临床阶段（II期10个，I期2个）。所有12个未甲基化的样品均显示hMLH1阳性染色。多元逻辑回归分析显示hMLH1基因的甲基化和OSCC早期阶段的OR为16.54（IC 95％：1.69-161.68，p = 0.016），并通过性别和烟草使用进行了调整。这项研究表明，在大多数早期病例和大约一半的晚期病例中，发生hMLH1启动子甲基化的频率很高。提出hMLH1启动子甲基化是在肿瘤进展期间维持的早期事件。

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