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首页> 外文期刊>Osteoarthritis and cartilage >Development and remodeling of engineered cartilage-explant composites in vitro and in vivo.
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Development and remodeling of engineered cartilage-explant composites in vitro and in vivo.

机译：在体外和体内开发和改造工程软骨-植入物复合材料。

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OBJECTIVE: Development and remodeling of engineered cartilage-explant composites were studied in vitro and in vivo. DESIGN: Individual and interactive effects of cell chondrogenic potential (primary or fifth passage bovine calf chondrocytes), scaffold degradation rate (hyaluronan benzyl ester or polyglycolic acid), and adjacent tissue cell activity and architecture (vital trabecular bone (VB), articular cartilage (AC), devitalized bone (DB) or digested cartilage (DC)) were evaluated over 8 weeks in vitro (bioreactor cultures) and in vivo (ectopic implants). RESULTS: In vitro, significant effects of cell type on construct adhesive strength (P<0.001) and scaffold type on adhesive strength (P<0.001), modulus (P=0.014), glycosaminoglycans (GAG) (P<0.001), and collagen (P=0.039) were observed. Chondrogenesis was best when the scaffold degradation rate matched the extracellular matrix deposition rate. In vivo, adjacent tissue type affected adhesive strength (P<0.001), modulus (P<0.001), and GAG (P<0.001) such that 8-week values obtained for bone (VB and DB) were higher than for cartilage (AC). In the AC/construct group, chondrogenesis appeared attenuated in the region of the construct close to the AC. In contrast, in the VB/construct group, a 500 microm thick zone of mature hyaline-like cartilage formed at the interface, and signs of active remodeling were present in the bone that included osteoclastic and osteoblastic activity and trabecular rebuttressing; these features were not present in the DB group or in vitro. CONCLUSIONS: Development and remodeling of composites based on engineered cartilage were mediated in vitro by cell chondrogenic potential and scaffold degradation rate, and in vivo by type of adjacent tissue and time.
机译：目的：在体外和体内研究工程化的软骨-移植物复合材料的开发和重塑。设计：细胞软骨形成电位（初次或第五代牛小牛软骨细胞），支架降解速率（透明质酸苄酯或聚乙醇酸）以及邻近组织细胞的活动和结构（小梁骨（VB），关节软骨（ AC），失活的骨骼（DB）或消化的软骨（DC））在体外（生物反应器培养物）和体内（异位植入物）进行了8周的评估。结果：在体外，细胞类型对构建体粘附强度（P <0.001）和支架类型对粘附强度（P <0.001），模量（P = 0.014），糖胺聚糖（GAG）（P <0.001）和胶原蛋白的显着影响（P ＝ 0.039）被观察到。当支架降解速率与细胞外基质沉积速率匹配时，软骨形成最好。在体内，相邻组织类型会影响粘合强度（P <0.001），模量（P <0.001）和GAG（P <0.001），以使骨骼（VB和DB）的8周值高于软骨（AC） ）。在AC /构建体组中，在靠近AC的构建体区域中软骨形成似乎减弱。相比之下，在VB /构建体组中，在界面处形成了一个500微米厚的成熟透明样软骨区域，并且在骨骼中出现了主动重塑的迹象，包括破骨和成骨活性以及小梁支撑。这些特征在DB组或体外均不存在。结论：基于工程软骨的复合材料的开发和重塑在体外是通过细胞软骨形成电位和支架降解速率来介导的，在体内是通过邻近组织的类型和时间来介导的。

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