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首页> 外文期刊>Osteoarthritis and cartilage >The short-term therapeutic effect of recombinant human bone morphogenetic protein-2 on collagenase-induced lumbar facet joint osteoarthritis in rats.
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The short-term therapeutic effect of recombinant human bone morphogenetic protein-2 on collagenase-induced lumbar facet joint osteoarthritis in rats.

机译：重组人骨形态发生蛋白2对胶原酶诱导的腰椎小关节骨关节炎的短期治疗作用。

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OBJECTIVE: To determine whether an intra-articular injection of recombinant human bone morphogenetic protein-2 (rhBMP-2) alleviates cartilage degradation in a rat model of osteoarthritis (OA) of the lumbar facet joint. METHOD: The right-side facet joint OA model was created by an intra-articular injection of collagenase (type II) 2 weeks before treatment. The OA rats were divided into four groups: (1) no treatment, or intra-articular injection of either (2) saline, (3) rhBMP-2 10ng, or (4) rhBMP-2 100ng. The left-side facet joint served as the normal control. At 3 and 6 weeks after treatment, histological analyses were performed on the cartilage, synovium, subchondral bone and bone marrow. The cartilage and synovium were graded using a modified Mankin score and a synovium score system. Extracellular type II collagen was evaluated by immunohistochemistry. RESULTS: Intra-articular injection of collagenase causes OA-like changes in the facet joint. OA rats treated with rhBMP-2 at both dosages tested showed reduced severity of their cartilage lesions compared with untreated and saline-treated groups. There was a statistically significant difference in the modified Mankin score compared to the untreated and saline-treated groups. However, some rhBMP-2-treated rats at the higher dose (100ng) showed, as a side effect, joint space obliteration caused by cartilage overgrowth. Also OA rats treated with 100ng of rhBMP-2 displayed a significant synovium reaction at 3 weeks compared with that in other groups. Immunohistochemical analysis showed that treatment with rhBMP-2 significantly increased the content of type II collagen. CONCLUSION: This study demonstrates the potential efficacy of rhBMP-2 in the alleviation of arthritic changes in a rat model of OA of the lumbar facet joint. However, treatment with a high dosage of rhBMP-2 caused adverse side effects in some animals.
机译：目的：确定关节内注射重组人骨形态发生蛋白2（rhBMP-2）是否能减轻腰椎小关节骨关节炎（OA）大鼠模型的软骨降解。方法：在治疗前2周通过关节内注射胶原酶（II型）建立右侧小关节OA模型。 OA大鼠分为四组：（1）不治疗，或关节内注射（2）盐水，（3）rhBMP-2 10ng或（4）rhBMP-2 100ng。左侧小关节为正常对照。治疗后第3和第6周，对软骨，滑膜，软骨下骨和骨髓进行组织学分析。使用改良的Mankin评分和滑膜评分系统对软骨和滑膜评分。通过免疫组织化学评估细胞外II型胶原。结果：关节内注射胶原酶会导致小关节的OA样变化。与未治疗组和生理盐水治疗组相比，两种剂量的rhBMP-2治疗的OA大鼠的软骨损伤严重程度均降低。与未治疗组和生理盐水治疗组相比，改良的Mankin评分存在统计学上的显着差异。但是，一些用高剂量（100ng）的rhBMP-2处理的大鼠显示出由软骨过度生长引起的关节间隙闭塞是一种副作用。与其他组相比，用100ng rhBMP-2处理的OA大鼠在第3周也表现出明显的滑膜反应。免疫组织化学分析显示，用rhBMP-2处理可显着增加II型胶原的含量。结论：本研究证明了rhBMP-2在减轻腰椎小关节OA模型大鼠关节炎方面的潜在疗效。但是，在一些动物中，高剂量的rhBMP-2处理会引起不利的副作用。

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