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首页> 外文期刊>Osteoarthritis and cartilage >Do the matrix degrading enzymes cathepsins B and D increase following a high intensity exercise regime?
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Do the matrix degrading enzymes cathepsins B and D increase following a high intensity exercise regime?

机译：高强度运动后，基质降解酶组织蛋白酶B和D是否增加？

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OBJECTIVE: It has been shown by others that levels of matrix degrading enzymes are increased in osteoarthritis (OA) and so are proposed to be involved in the aetiopathogenesis of the disease, including exercise-associated OA. Therefore we hypothesised that cathepsin B and cathepsin D were increased in cartilage samples previously shown to have early stage OA from 2-year-old Thoroughbred horses, euthanased for reasons other than this study, that had a history of 19-week high intensity exercise (n=6) compared to age and sex-matched horses with a history of low intensity exercise (n=6). METHODS: Cartilage samples were used from four specific sites within the carpal joints. Standard immunolocalisation protocols and blind counting of positive and negative cells within the articular surface, mid-zone and deep zone (DZ) were used to test our hypothesis. RESULTS: A high intensity exercise regime did not significantly alter the number of chondrocytes positive for cathepsin B, whereas a significant decrease was found for cathepsin D in the DZ, indicating that these enzymes are regulated differently by mechanical loading. Furthermore, cathepsin D varied according to the topographical location within the joint, reflecting biomechanical differences experienced during a high compared to a low intensity exercise regime. CONCLUSION: This study disproves our hypothesis that cathepsins B and D are increased following a high intensity exercise regime unlike that reported for other matrix enzymes.
机译：目的：其他人已经表明，骨关节炎（OA）中基质降解酶的水平增加，因此被认为与该病的发病机制有关，包括运动相关的OA。因此，我们假设在先前显示的具有2周龄纯种马早期OA的软骨样品中，组织蛋白酶B和组织蛋白酶D有所增加。 n = 6）与具有低强度运动史的年龄和性别匹配的马相比（n = 6）。方法：从腕关节内的四个特定部位使用软骨样品。使用标准的免疫定位方案以及在关节表面，中部区域和深部区域（DZ）内对阳性和阴性细胞进行盲点计数来检验我们的假设。结果：高强度运动方案并没有显着改变组织蛋白酶B阳性的软骨细胞数量，而在DZ中发现组织蛋白酶D的显着减少，表明这些酶受机械负荷的调节不同。此外，组织蛋白酶D根据关节内的地形位置而变化，反映出与低强度运动方案相比在高强度运动期间经历的生物力学差异。结论：这项研究证明了我们的假设，即组织蛋白酶B和D在高强度运动后会增加，这与其他基质酶的报道不同。

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